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Journal of Virology, August 2002, p. 7968-7975, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.7968-7975.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparative Sequence Analysis of the Largest E1A Proteins of Human and Simian Adenoviruses

Nikita Avvakumov,1 Russ Wheeler,2 Jean Claude D'Halluin,3 and Joe S. Mymryk1,4,5*

Departments of Microbiology and Immunology,1 Pharmacology and Toxicology,4 Oncology, London Regional Cancer Centre, The University of Western Ontario, London, Ontario, Canada N6A 4L6,5 London Laboratory Services Group, St. Joseph's Hospital, London, Ontario, Canada N6A 4V2,2 INSERM UR524, 59045 Lille, France3

Received 19 March 2002/ Accepted 7 May 2002

The early region 1A (E1A) gene is the first gene expressed after infection with adenovirus and has been most extensively characterized in human adenovirus type 5 (hAd5). The E1A proteins interact with numerous cellular regulatory proteins, influencing a variety of transcriptional and cell cycle events. For this reason, these multifunctional proteins have been useful as tools for dissecting pathways regulating cell growth and gene expression. Despite the large number of studies using hAd5 E1A, relatively little is known about the function of the E1A proteins of other adenoviruses. In 1985, a comparison of E1A sequences from three human and one simian adenovirus identified three regions with higher overall levels of sequence conservation designated conserved regions (CR) 1, 2, and 3. As expected, these regions are critical for a variety of E1A functions. Since that time, the sequences of several other human and simian adenovirus E1A proteins have been determined. Using these, and two additional sequences that we determined, we report here a detailed comparison of the sequences of 15 E1A proteins representing each of the six hAd subgroups and several simian adenoviruses. These analyses refine the positioning of CR1, 2, and 3; define a fourth CR located near the carboxyl terminus of E1A; and suggest several new functions for E1A.

* Corresponding author. Mailing address: London Regional Cancer Centre, 790 Commissioners Rd. East, London, Ontario, Canada N6A 4L6. Phone: (519) 685-8617. Fax: (519) 685-8616. E-mail address: jmymryk{at}uwo.ca.

Journal of Virology, August 2002, p. 7968-7975, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.7968-7975.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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