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Journal of Virology, August 2002, p. 7890-7896, Vol. 76, No. 15
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.15.7890-7896.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Contains Two Functional Lytic Origins of DNA Replication

David P. AuCoin, Kelly S. Colletti, Yiyang Xu, Sylvia A. Cei, and Gregory S. Pari*

Department of Microbiology and Cell and Molecular Biology Program, University of Nevada School of Medicine, Reno, Nevada 89557

Received 18 March 2002/ Accepted 25 April 2002

We used a transient-transfection replication assay to identify two functional copies of the human herpesvirus 8 (HHV8) lytic origin of DNA replication (oriLyt). BCLB-1 cells were transfected with HHV8 subgenomic fragments containing the putative lytic origin along with a plasmid expressing viral transactivator open reading frame (ORF) 50. The HHV8 left-end oriLyt (oriLyt-L) lies between ORFs K4.2 and K5 and is composed of a region encoding various transcription factor binding sites and an A+T-rich region and a G+C repeat region. The right-end oriLyt (oriLyt-R) maps between ORF 69 and vFLIP, a region similar to the RRV oriLyt, and is an inverted duplication of oriLyt-L.

* Corresponding author. Mailing address: University of Nevada, Reno, School of Medicine/Dept. of Microbiology, Howard Bldg., Reno, NV 89557. Phone: (775) 784-4824. Fax: (775) 327-2332. E-mail: gpari{at}med.unr.edu.

Journal of Virology, August 2002, p. 7890-7896, Vol. 76, No. 15
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.15.7890-7896.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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