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Journal of Virology, August 2002, p. 7694-7704, Vol. 76, No. 15
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.15.7694-7704.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Determination of the Structure of a Decay Accelerating Factor-Binding Clinical Isolate of Echovirus 11 Allows Mapping of Mutants with Altered Receptor Requirements for Infection

Amanda D. Stuart,1 Thomas A. McKee,1,{dagger} Pamela A. Williams,2,{ddagger} Chris Harley,1 Shuo Shen,1 David I. Stuart,3 T. David K. Brown,1 and Susan M. Lea2*

Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP,1 Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU,2 Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, United Kingdom3

Received 5 March 2001/ Accepted 18 April 2002

We have used X-ray crystallography to determine the structure of a decay accelerating factor (DAF)-binding, clinic-derived isolate of echovirus 11 (EV11-207). The structures of the capsid proteins closely resemble those of capsid proteins of other picornaviruses. The structure allows us to interpret a series of amino acid changes produced by passaging EV11-207 in different cell lines as highlighting the locations of multiple receptor-binding sites on the virion surface. We suggest that a DAF-binding site is located at the fivefold axes of the virion, while the binding site for a distinct but as yet unidentified receptor is located within the canyon surrounding the virion fivefold axes.

* Corresponding author. Mailing address: Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Rd., Oxford OX1 3QU, United Kingdom. Phone: 44 (0) 1865 275181. Fax: 44 (0) 1865 275182. E-mail: susan{at}biop.ox.ac.uk.

{dagger} Present address: Division de Pathologie Clinique, 1211 Geneva 4, Switzerland.

{ddagger} Present address: ASTEX-Technology, Cambridge CB4 0WE, United Kingdom.

Journal of Virology, August 2002, p. 7694-7704, Vol. 76, No. 15
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.15.7694-7704.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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