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Journal of Virology, August 2002, p. 7578-7586, Vol. 76, No. 15
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.15.7578-7586.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Viral Gene Expression Patterns in Human Herpesvirus 6B-Infected T Cells

Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark

Received 7 March 2002/ Accepted 30 April 2002

Herpesvirus gene expression is divided into immediate-early (IE) or genes, early (E) or ß genes, and late (L) or genes on the basis of temporal expression and dependency on other gene products. By using real-time PCR, we have investigated the expression of 35 human herpesvirus 6B (HHV-6B) genes in T cells infected by strain PL-1. Kinetic analysis and dependency on de novo protein synthesis and viral DNA polymerase activity suggest that the HHV-6B genes segregate into six separate kinetic groups. The genes expressed early (groups I and II) and late (groups V and VI) corresponded well with IE and L genes, whereas the intermediate groups III and IV contained E and L genes. Although HHV-6B has characteristics similar to those of other roseoloviruses in its overall gene regulation, we detected three B-variant-specific IE genes. Moreover, genes that were independent of de novo protein synthesis clustered in an area of the viral genome that has the lowest identity to the HHV-6A variant. The organization of IE genes in an area of the genome that differs from that of HHV-6A underscores the distinct differences between HHV-6B and HHV-6A and may provide a basis for further molecular and immunological analyses to elucidate their different biological behaviors.

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