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Journal of Virology, July 2002, p. 7334-7342, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.7334-7342.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of Tat-Encoding Bicistronic Human Immunodeficiency Virus Type 1 Gene Transfer Vectors in Primary Canine Bone Marrow Mononuclear Cells

Narasimhachar Srinivasakumar,* Michail Zaboikin, Tatiana Zaboikina, and Friedrich Schuening

Division of Hematology/Oncology, Department of Medicine, Vanderbilt University, Nashville, Tennessee

Received 27 November 2001/ Accepted 2 April 2002

Tat-encoding human immunodeficiency virus type 1 (HIV-1) gene transfer vectors were evaluated in primary canine bone marrow mononuclear cells. Tat vectors provided higher levels of gene expression than vectors with internal promoters. The HIV-1 vector was also more efficient than Moloney murine leukemia virus (MoMLV) vectors for transduction of canine bone marrow mononuclear cells in vitro. Transplantation experiments in dogs with transduced autologous marrow cells confirmed the superiority of HIV-1 vectors over MoMLV vectors for gene transfer into canine bone marrow cells. Tat vectors may be useful not only for providing high levels of therapeutic gene expression in hematopoietic cells but also for study of the biological effects of Tat in those tissues in the canine model.

* Corresponding author. Mailing address: Division of Hematology/Oncology, Department of Medicine, Vanderbilt University, 777 Preston Research Building, 2220 Pierce Ave., Nashville, TN 37232-6305. Phone: (615) 936-2134. Fax: (615) 936-3853. E-mail: narasimhachar.srinivasakumar{at}mcmail.vanderbilt.edu.

Journal of Virology, July 2002, p. 7334-7342, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.7334-7342.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schuening, F. G., Srinivasakumar, N., Zaboikin, M. M., Zaboikina, T. N. (2005). HIV-1 Vector Mediated Expression of Human B-Domain Deleted FVIII in Primary Canine Marrow Mononuclear Cells.. ASH ANNUAL MEETING ABSTRACTS 106: 5524-5524 [Abstract]  


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