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Journal of Virology, July 2002, p. 7276-7292, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.7276-7292.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of the Neoplastically Transformed Cells in Marek's Disease Herpesvirus-Induced Lymphomas: Recognition by the Monoclonal Antibody AV37

Shane C. Burgess* and T. Fred Davison

Division of Immunology and Pathology, Institute for Animal Health, Compton, United Kingdom

Received 3 January 2002/ Accepted 18 April 2002

Understanding the interactions between herpesviruses and their host cells and also the interactions between neoplastically transformed cells and the host immune system is fundamental to understanding the mechanisms of herpesvirus oncology. However, this has been difficult as no animal models of herpesvirus-induced oncogenesis in the natural host exist in which neoplastically transformed cells are also definitively identified and may be studied in vivo. Marek's disease (MD) herpesvirus (MDV) of poultry, although a recognized natural oncogenic virus causing T-cell lymphomas, is no exception. In this work, we identify for the first time the neoplastically transformed cells in MD as the CD4+ major histocompatibility complex (MHC) class Ihi, MHC class IIhi, interleukin-2 receptor {alpha}-chain-positive, CD28lo/-, phosphoprotein 38-negative (pp38-), glycoprotein B-negative (gB-), {alpha}ß T-cell-receptor-positive (TCR+) cells which uniquely overexpress a novel host-encoded extracellular antigen that is also expressed by MDV-transformed cell lines and recognized by the monoclonal antibody (MAb) AV37. Normal uninfected leukocytes and MD lymphoma cells were isolated directly ex vivo and examined by flow cytometry with MAb recognizing AV37, known leukocyte antigens, and MDV antigens pp38 and gB. CD28 mRNA was examined by PCR. Cell cycle distribution and in vitro survival were compared for each lymphoma cell population. We demonstrate for the first time that the antigen recognized by AV37 is expressed at very low levels by small minorities of uninfected leukocytes, whereas particular MD lymphoma cells uniquely express extremely high levels of the AV37 antigen; the AV37hi MD lymphoma cells fulfill the accepted criteria for neoplastic transformation in vivo (protection from cell death despite hyperproliferation, presence in all MD lymphomas, and not supportive of MDV production); the lymphoma environment is essential for AV37+ MD lymphoma cell survival; pp38 is an antigen expressed during MDV-productive infection and is not expressed by neoplastically transformed cells in vivo; AV37+ MD lymphoma cells have the putative immune evasion mechanism of CD28 down-regulation; AV37hi peripheral blood leukocytes appear early after MDV infection in both MD-resistant and -susceptible chickens; and analysis of TCR variable ß chain gene family expression suggests that MD lymphomas have polyclonal origins. Identification of the neoplastically transformed cells in MD facilitates a detailed understanding of MD pathogenesis and also improves the utility of MD as a general model for herpesvirus oncology.

* Corresponding author. Present address: Department of Research and Basic Science, Mississippi State University, College of Veterinary Medicine, Wise Center/Spring St., P.O. Box 6100, Mississippi State, MS 39762-6100. Phone: (662) 325-1239. Fax: (662) 325-1031. E-mail: burgess{at}cvm.msstate.edu.

Journal of Virology, July 2002, p. 7276-7292, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.7276-7292.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Burgess, S. C., Young, J. R., Baaten, B. J. G., Hunt, L., Ross, L. N. J., Parcells, M. S., Kumar, P. M., Tregaskes, C. A., Lee, L. F., Davison, T. F. (2004). Marek's disease is a natural model for lymphomas overexpressing Hodgkin's disease antigen (CD30). Proc. Natl. Acad. Sci. USA 101: 13879-13884 [Abstract] [Full Text]  
  • Barrow, A. D., Burgess, S. C., Baigent, S. J., Howes, K., Nair, V. K. (2003). Infection of macrophages by a lymphotropic herpesvirus: a new tropism for Marek's disease virus. J. Gen. Virol. 84: 2635-2645 [Abstract] [Full Text]  


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