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Journal of Virology, July 2002, p. 6701-6709, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6701-6709.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Asymmetric Requirement for Cholesterol in Receptor-Bearing but Not Envelope-Bearing Membranes for Fusion Mediated by Ecotropic Murine Leukemia Virus

Xiongbin Lu,,{dagger} Ying Xiong, and Jonathan Silver*

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892

Received 9 October 2001/ Accepted 22 March 2002

We show that fusion mediated by ecotropic murine leukemia virus envelope is dependent on cholesterol in receptor-bearing membranes. The effect is >10 times larger in insect cells than mammalian cells, probably because the former can be more extensively depleted of cholesterol. The fact that cholesterol is apparently not needed in envelope-bearing membranes suggests that it plays a role in an asymmetric step in membrane fusion and argues against a class of models in which cholesterol is important in symmetric fusion intermediates. The insect cell system has promise for clarifying the role of membrane rafts in other aspects of cell physiology.

* Corresponding author. Mailing address: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 4, Room 336, Bethesda, MD 20892. Phone: (301) 496-3653. Fax: (301) 402-0226. E-mail: jsilver{at}nih.gov.

{dagger} Present address: Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030.

Journal of Virology, July 2002, p. 6701-6709, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6701-6709.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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