This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Serhan, F.
Right arrow Articles by Duisit, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Serhan, F.
Right arrow Articles by Duisit, G.

 Previous Article  |  Next Article 

Journal of Virology, July 2002, p. 6609-6617, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6609-6617.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Producer Cell-Dependent Restriction of Murine Leukemia Virus Replication

Fatima Serhan, Nathalie Jourdan, Sylvie Saleun, Philippe Moullier, and Ghislaine Duisit*

Laboratoire de Thérapie Génique, INSERM ERM 0-105, CHU Hotel Dieu, 44035 Nantes Cedex 01, France

Received 8 February 2002/ Accepted 21 March 2002

We previously reported that the human bronchocarcinoma cell line A549 produces poorly infectious gibbon ape leukemia virus-pseudotyped Moloney murine leukemia virus (MLV). In contrast, similar amounts of virions recovered from human fibrosarcoma HT1080 cells result in 10-fold-higher transduction rates (G. Duisit, A. Salvetti, P. Moullier, and F. Cosset, Hum. Gene Ther. 10:189-200, 1999). We have now extended this initial observation to other type-C envelope (Env) pseudotypes and analyzed the mechanism involved. Structural and morphological analysis showed that viral particles recovered from A549 (A549-MLV) and HT1080 (HT1080-MLV) cells were normal and indistinguishable from each other. They expressed equivalent levels of mature Env proteins and bound similarly to the target cells. Furthermore, incoming particles reached the cytosol and directed the synthesis of linear viral DNA equally efficiently. However, almost no detectable circular DNAs could be detected in A549-MLV-infected cells, indicating that the block of infection resulted from defective nuclear translocation of the preintegration complex. Interestingly, pseudotyping of A549-MLV with vesicular stomatitis virus glycoprotein G restored the amount of circular DNA forms as well as the transduction rates to HT1080-MLV levels, suggesting that the postentry blockage could be overcome by endocytic delivery of the core particles downstream of the restriction point. Thus, in contrast to the previously described target cell-dependent Fv-1 (or Fv1-like) restriction in mammalian cells (P. Pryciak and H. E. Varmus, J. Virol. 66:5959-5966, 1992; G. Towers, M. Bock, S. Martin, Y. Takeuchi, J. P. Stoye, and O. Danos, Proc. Natl. Acad. Sci. USA 97:12295-12299, 2000), we report here a new restriction of MLV replication that relies only on the producer cell type.

* Corresponding author. Mailing address: INSERM ERM 0-105, CHU Hôtel-Dieu, 30, bvd Jean Monnet, 44035 Nantes Cedex 01, France. Phone: (33) 240087490. Fax: (33) 240087491. E-mail: gduisit{at}sante.univ-nantes.fr.

Journal of Virology, July 2002, p. 6609-6617, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6609-6617.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Serhan, F., Penaud, M., Petit, C., Leste-Lasserre, T., Trajcevski, S., Klatzmann, D., Duisit, G., Sonigo, P., Moullier, P. (2004). Early Detection of a Two-Long-Terminal-Repeat Junction Molecule in the Cytoplasm of Recombinant Murine Leukemia Virus-Infected Cells. J. Virol. 78: 6190-6199 [Abstract] [Full Text]  
  • Schmitz, C., Marchant, D., Neil, S. J. D., Aubin, K., Reuter, S., Dittmar, M. T., McKnight, A. (2004). Lv2, a Novel Postentry Restriction, Is Mediated by both Capsid and Envelope. J. Virol. 78: 2006-2016 [Abstract] [Full Text]  
  • Berthoux, L., Towers, G. J., Gurer, C., Salomoni, P., Pandolfi, P. P., Luban, J. (2003). As2O3 Enhances Retroviral Reverse Transcription and Counteracts Ref1 Antiviral Activity. J. Virol. 77: 3167-3180 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»