This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Iturriza Gómara, M. Articles by Gray, J. Search for Related Content PubMed PubMed Citation Articles by Iturriza Gómara, M. Articles by Gray, J.

 Previous Article  |  Next Article 

Journal of Virology, July 2002, p. 6596-6601, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6596-6601.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of VP6 Genes of Human Rotavirus Isolates: Correlation of Genogroups with Subgroups and Evidence of Independent Segregation

Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, United Kingdom

Received 10 December 2001/ Accepted 28 March 2002

A reverse transcription-PCR (RT-PCR) was established to amplify a 379-bp cDNA fragment (nucleotides 747 to 1126, coding for amino acids 241 to 367) of the VP6 gene of group A rotaviruses associated with subgroup (SG) specificity. Thirty-eight human rotavirus strains characterized with SG-specific monoclonal antibodies were subjected to VP6-specific RT-PCR, and PCR amplicons were used for sequencing. Nucleic acid sequencing and phylogenetic analysis of the VP6 amplicons revealed two clusters, or genogroups. Two genetic lineages were distinguished within genogroup I, consisting of strains serologically characterized as SG I, and three genetic lineages were distinguished within genogroup II, composed of strains serologically characterized as SG II, SG I + II, and SG non-I, non-II. Subgrouping of rotaviruses by means of serological methods may result in strains not being assigned the correct SG or in a failure of strains to subgroup. Molecular characterization of the SG-defining region of VP6 provided evidence for independent segregation of the rotavirus genes encoding VP4, VP6, and VP7.

This article has been cited by other articles:

• Steyer, A., Poljsak-Prijatelj, M., Barlic-Maganja, D., Marin, J. (2008). Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment. J. Gen. Virol. 89: 1690-1698 [Abstract] [Full Text]  
• Stockman, L. J., Staat, M. A., Holloway, M., Bernstein, D. I., Kerin, T., Hull, J., Yee, E., Gentsch, J., Parashar, U. D. (2008). Optimum Diagnostic Assay and Clinical Specimen for Routine Rotavirus Surveillance. J. Clin. Microbiol. 46: 1842-1843 [Abstract] [Full Text]  
• Matthijnssens, J., Ciarlet, M., Heiman, E., Arijs, I., Delbeke, T., McDonald, S. M., Palombo, E. A., Iturriza-Gomara, M., Maes, P., Patton, J. T., Rahman, M., Van Ranst, M. (2008). Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains. J. Virol. 82: 3204-3219 [Abstract] [Full Text]  
• De Grazia, S., Giammanco, G. M., Martella, V., Ramirez, S., Colomba, C., Cascio, A., Arista, S. (2008). Rare AU-1-Like G3P[9] Human Rotaviruses with a Kun-Like NSP4 Gene Detected in Children with Diarrhea in Italy. J. Clin. Microbiol. 46: 357-360 [Abstract] [Full Text]  
• Tcheremenskaia, O., Marucci, G., De Petris, S., Ruggeri, F. M., Dovecar, D., Sternak, S. L., Matyasova, I., Dhimolea, M. K., Mladenova, Z., Fiore, L., and the Rotavirus Study Group, (2007). Molecular Epidemiology of Rotavirus in Central and Southeastern Europe. J. Clin. Microbiol. 45: 2197-2204 [Abstract] [Full Text]  
• Araujo, I. T., Heinemann, M. B., Mascarenhas, J. D. P., Assis, R. M. S., Fialho, A. M., Leite, J. P. G. (2007). Molecular analysis of the NSP4 and VP6 genes of rotavirus strains recovered from hospitalized children in Rio de Janeiro, Brazil. J Med Microbiol 56: 854-859 [Abstract] [Full Text]  
• Mascarenhas, J. D. P., Leite, J. P. G., Lima, J. C., Heinemann, M. B., Oliveira, D. S., Araujo, I. T., Soares, L. S., Gusmao, R. H. P., Gabbay, Y. B., Linhares, A. C. (2007). Detection of a neonatal human rotavirus strain with VP4 and NSP4 genes of porcine origin. J Med Microbiol 56: 524-532 [Abstract] [Full Text]  
• Park, S.-H., Saif, L. J., Jeong, C., Lim, G.-K., Park, S.-I., Kim, H.-H., Park, S.-J., Kim, Y.-J., Jeong, J.-H., Kang, M.-I., Cho, K.-O. (2006). Molecular Characterization of Novel G5 Bovine Rotavirus Strains. J. Clin. Microbiol. 44: 4101-4112 [Abstract] [Full Text]  
• Matthijnssens, J., Rahman, M., Martella, V., Xuelei, Y., De Vos, S., De Leener, K., Ciarlet, M., Buonavoglia, C., Van Ranst, M. (2006). Full genomic analysis of human rotavirus strain b4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission.. J. Virol. 80: 3801-3810 [Abstract] [Full Text]  
• Gallimore, C. I., Taylor, C., Gennery, A. R., Cant, A. J., Galloway, A., Iturriza-Gomara, M., Gray, J. J. (2006). Environmental Monitoring for Gastroenteric Viruses in a Pediatric Primary Immunodeficiency Unit. J. Clin. Microbiol. 44: 395-399 [Abstract] [Full Text]  
• McNeal, M. M., Sestak, K., Choi, A. H.-C., Basu, M., Cole, M. J., Aye, P. P., Bohm, R. P., Ward, R. L. (2005). Development of a Rotavirus-Shedding Model in Rhesus Macaques, Using a Homologous Wild-Type Rotavirus of a New P Genotype. J. Virol. 79: 944-954 [Abstract] [Full Text]  
• Iturriza Gomara, M., Kang, G., Mammen, A., Jana, A. K., Abraham, M., Desselberger, U., Brown, D., Gray, J. (2004). Characterization of G10P[11] Rotaviruses Causing Acute Gastroenteritis in Neonates and Infants in Vellore, India. J. Clin. Microbiol. 42: 2541-2547 [Abstract] [Full Text]  
• Martella, V., Ciarlet, M., Pratelli, A., Arista, S., Terio, V., Elia, G., Cavalli, A., Gentile, M., Decaro, N., Greco, G., Cafiero, M. A., Tempesta, M., Buonavoglia, C. (2003). Molecular Analysis of the VP7, VP4, VP6, NSP4, and NSP5/6 Genes of a Buffalo Rotavirus Strain: Identification of the Rare P[3] Rhesus Rotavirus-Like VP4 Gene Allele. J. Clin. Microbiol. 41: 5665-5675 [Abstract] [Full Text]  
• Iturriza-Gomara, M., Anderton, E., Kang, G., Gallimore, C., Phillips, W., Desselberger, U., Gray, J. (2003). Evidence for Genetic Linkage between the Gene Segments Encoding NSP4 and VP6 Proteins in Common and Reassortant Human Rotavirus Strains. J. Clin. Microbiol. 41: 3566-3573 [Abstract] [Full Text]  
• Martella, V., Terio, V., Del Gaudio, G., Gentile, M., Fiorente, P., Barbuti, S., Buonavoglia, C. (2003). Detection of the Emerging Rotavirus G9 Serotype at High Frequency in Italy. J. Clin. Microbiol. 41: 3960-3963 [Abstract] [Full Text]