This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vidalain, P.-O.
Right arrow Articles by Valentin, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vidalain, P.-O.
Right arrow Articles by Valentin, H.

Next Article 

Journal of Virology, July 2002, p. 6415-6424, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6415-6424.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Interferons Mediate Terminal Differentiation of Human Cortical Thymic Epithelial Cells

Pierre-Olivier Vidalain,1 David Laine,1 Yona Zaffran,1 Olga Azocar,1 Christine Servet-Delprat,1 T. Fabian Wild,2 Chantal Rabourdin-Combe,1 and Hélène Valentin1*

Laboratoire d'Immunobiologie Fondamentale et Clinique, INSERM U503,1 Immunity and Vaccination, INSERM U404, 69365 Lyon Cedex 07, France2

Received 11 December 2001/ Accepted 28 March 2002

In the thymus, epithelial cells comprise a heterogeneous population required for the generation of functional T lymphocytes, suggesting that thymic epithelium disruption by viruses may compromise T-cell lymphopoiesis in this organ. In a previous report, we demonstrated that in vitro, measles virus induced differentiation of cortical thymic epithelial cells as characterized by (i) cell growth arrest, (ii) morphological and phenotypic changes, and (iii) apoptotis as a final step of this process. In the present report, we have analyzed the mechanisms involved. First, measles virus-induced differentiation of thymic epithelial cells is shown to be strictly dependent on beta interferon (IFN-ß) secretion. In addition, transfection with double-stranded RNA, a common intermediate of replication for a broad spectrum of viruses, is reported to similarly mediate thymic epithelial cell differentiation through IFN-ß induction. Finally, we demonstrated that recombinant IFN-{alpha}, IFN-ß, or IFN-{gamma} was sufficient to induce differentiation and apoptosis of uninfected thymic epithelial cells. These observations suggested that interferon secretion by either infected cells or activated leukocytes, such as plasmacytoid dendritic cells or lymphocytes, may induce thymic epithelium disruption in a pathological context. Thus, we have identified a new mechanism that may contribute to thymic atrophy and altered T-cell lymphopoiesis associated with many infections.

* Corresponding author. Mailing address: INSERM U503, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07, France. Phone: (33) 4 37 28 23 77. Fax: (33) 4 37 28 23 41. E-mail: valentin{at}cervi-lyon.inserm.fr.

Journal of Virology, July 2002, p. 6415-6424, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6415-6424.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Anz, D., Thaler, R., Stephan, N., Waibler, Z., Trauscheid, M. J., Scholz, C., Kalinke, U., Barchet, W., Endres, S., Bourquin, C. (2009). Activation of Melanoma Differentiation-Associated Gene 5 Causes Rapid Involution of the Thymus. J. Immunol. 182: 6044-6050 [Abstract] [Full Text]  
  • Demoulins, T., Abdallah, A., Kettaf, N., Baron, M.-L., Gerarduzzi, C., Gauchat, D., Gratton, S., Sekaly, R.-P. (2008). Reversible Blockade of Thymic Output: An Inherent Part of TLR Ligand-Mediated Immune Response. J. Immunol. 181: 6757-6769 [Abstract] [Full Text]  
  • Kisand, K., Link, M., Wolff, A. S. B., Meager, A., Tserel, L., Org, T., Murumagi, A., Uibo, R., Willcox, N., Trebusak Podkrajsek, K., Battelino, T., Lobell, A., Kampe, O., Lima, K., Meloni, A., Ergun-Longmire, B., Maclaren, N. K., Perheentupa, J., Krohn, K. J. E., Scott, H. S., Husebye, E. S., Peterson, P. (2008). Interferon autoantibodies associated with AIRE deficiency decrease the expression of IFN-stimulated genes. Blood 112: 2657-2666 [Abstract] [Full Text]  
  • Herschke, F., Plumet, S., Duhen, T., Azocar, O., Druelle, J., Laine, D., Wild, T. F., Rabourdin-Combe, C., Gerlier, D., Valentin, H. (2007). Cell-Cell Fusion Induced by Measles Virus Amplifies the Type I Interferon Response. J. Virol. 81: 12859-12871 [Abstract] [Full Text]  
  • Schmidlin, H., Dontje, W., Groot, F., Ligthart, S. J., Colantonio, A. D., Oud, M. E., Schilder-Tol, E. J., Spaargaren, M., Spits, H., Uittenbogaart, C. H., Blom, B. (2006). Stimulated plasmacytoid dendritic cells impair human T-cell development. Blood 108: 3792-3800 [Abstract] [Full Text]  
  • Strat, A., Gao, L., Utsuki, T., Cheng, B., Nuthalapaty, S., Mathis, J. M., Odaka, Y., Giordano, T. (2006). Specific and nontoxic silencing in mammalian cells with expressed long dsRNAs. Nucleic Acids Res 34: 3803-3810 [Abstract] [Full Text]  
  • Dontje, W., Schotte, R., Cupedo, T., Nagasawa, M., Scheeren, F., Gimeno, R., Spits, H., Blom, B. (2006). Delta-like1-induced Notch1 signaling regulates the human plasmacytoid dendritic cell versus T-cell lineage decision through control of GATA-3 and Spi-B. Blood 107: 2446-2452 [Abstract] [Full Text]  
  • Laine, D., Trescol-Biemont, M.-C., Longhi, S., Libeau, G., Marie, J. C., Vidalain, P.-O., Azocar, O., Diallo, A., Canard, B., Rabourdin-Combe, C., Valentin, H. (2003). Measles Virus (MV) Nucleoprotein Binds to a Novel Cell Surface Receptor Distinct from Fc{gamma}RII via Its C-Terminal Domain: Role in MV-Induced Immunosuppression. J. Virol. 77: 11332-11346 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»