This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thumfart, J. O.
Right arrow Articles by Meyers, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thumfart, J. O.
Right arrow Articles by Meyers, G.

 Previous Article  |  Next Article 

Journal of Virology, June 2002, p. 6398-6407, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6398-6407.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Feline Calicivirus: Recovery of Wild-Type and Recombinant Viruses after Transfection of cRNA or cDNA Constructs

Jörg Oliver Thumfart and Gregor Meyers*

Institute of Immunology, Federal Research Centre for Virus Diseases of Animals, D-72001 Tübingen, Germany

Received 17 October 2001/ Accepted 12 March 2002

The RNA genome of the vaccine strain 2024 of feline calicivirus was cloned as cDNA and analyzed by nucleotide sequencing. A full-length DNA copy of the viral genome was established and proved to be a source of infectious cRNA after in vitro transcription and RNA transfection. Virus could also be recovered when the DNA construct was introduced into cells containing phage T7 RNA polymerase that was provided by vaccinia virus MVA-T7. After insertion of the sequence encoding the green fluorescent protein into the structural protein-encoding region of the infectious cDNA clone, a defective replicon was recovered that was able to replicate autonomously and was packaged into virus particles when the structural proteins were provided in trans.

* Corresponding author. Mailing address: Federal Research Centre for Virus Diseases of Animals, P.O. Box 1149, D-72001 Tübingen, Germany. Phone: 49-7071-9670. Fax: 49-7071-967303. E-mail: gregor.meyers{at}tue.bfav.de.

Journal of Virology, June 2002, p. 6398-6407, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6398-6407.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Luttermann, C., Meyers, G. (2009). The importance of inter- and intramolecular base pairing for translation reinitiation on a eukaryotic bicistronic mRNA. Genes Dev. 23: 331-344 [Abstract] [Full Text]  
  • Liu, G., Ni, Z., Yun, T., Yu, B., Chen, L., Zhao, W., Hua, J., Chen, J. (2008). A DNA-launched reverse genetics system for rabbit hemorrhagic disease virus reveals that the VP2 protein is not essential for virus infectivity. J. Gen. Virol. 89: 3080-3085 [Abstract] [Full Text]  
  • Wei, C., Farkas, T., Sestak, K., Jiang, X. (2008). Recovery of Infectious Virus by Transfection of In Vitro-Generated RNA from Tulane Calicivirus cDNA. J. Virol. 82: 11429-11436 [Abstract] [Full Text]  
  • Chaudhry, Y., Skinner, M. A., Goodfellow, I. G. (2007). Recovery of genetically defined murine norovirus in tissue culture by using a fowlpox virus expressing T7 RNA polymerase. J. Gen. Virol. 88: 2091-2100 [Abstract] [Full Text]  
  • Luttermann, C., Meyers, G. (2007). A Bipartite Sequence Motif Induces Translation Reinitiation in Feline Calicivirus RNA. J. Biol. Chem. 282: 7056-7065 [Abstract] [Full Text]  
  • Ossiboff, R. J., Sheh, A., Shotton, J., Pesavento, P. A., Parker, J. S. L. (2007). Feline caliciviruses (FCVs) isolated from cats with virulent systemic disease possess in vitro phenotypes distinct from those of other FCV isolates. J. Gen. Virol. 88: 506-517 [Abstract] [Full Text]  
  • Liu, G., Zhang, Y., Ni, Z., Yun, T., Sheng, Z., Liang, H., Hua, J., Li, S., Du, Q., Chen, J. (2006). Recovery of infectious rabbit hemorrhagic disease virus from rabbits after direct inoculation with in vitro-transcribed RNA.. J. Virol. 80: 6597-6602 [Abstract] [Full Text]  
  • Asanaka, M., Atmar, R. L., Ruvolo, V., Crawford, S. E., Neill, F. H., Estes, M. K. (2005). Replication and packaging of Norwalk virus RNA in cultured mammalian cells. Proc. Natl. Acad. Sci. USA 102: 10327-10332 [Abstract] [Full Text]  
  • Sosnovtsev, S. V., Belliot, G., Chang, K.-O., Onwudiwe, O., Green, K. Y. (2005). Feline Calicivirus VP2 Is Essential for the Production of Infectious Virions. J. Virol. 79: 4012-4024 [Abstract] [Full Text]  
  • Chang, K.-O., Sosnovtsev, S. S., Belliot, G., Wang, Q., Saif, L. J., Green, K. Y. (2005). Reverse Genetics System for Porcine Enteric Calicivirus, a Prototype Sapovirus in the Caliciviridae. J. Virol. 79: 1409-1416 [Abstract] [Full Text]  
  • Mitra, T., Sosnovtsev, S. V., Green, K. Y. (2004). Mutagenesis of Tyrosine 24 in the VPg Protein Is Lethal for Feline Calicivirus. J. Virol. 78: 4931-4935 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»