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Journal of Virology, June 2002, p. 6121-6130, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6121-6130.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Restriction of Measles Virus RNA Synthesis by a Mouse Host Cell Line: trans-Complementation by Polymerase Components or a Human Cellular Factor(s)

Séverine Vincent,1 Isabelle Tigaud,2 Henriette Schneider,3 Christian J. Buchholz,3 Yusuke Yanagi,4 and Denis Gerlier1*

Immunité & Infections Virales, CNRS-UCBL UMR 5537, IFR62, Faculté de Médecine Lyon RTH Laennec, 69372 Lyon Cedex 08,1 Laboratoire de Cytogénétique Moléculaire, Hopital Edouard Herriot, 69437 Lyon, France,2 Institut für Molekularbiologie, Universität Zürich-Irchel, 8057 Zürich, Switzerland,3 Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan4

Received 7 December 2001/ Accepted 19 March 2002

The mouse epithelial MODE-K cell line expressing human CD46 or CD150 cellular receptors was found to be nonpermissive for measles virus (MV) replication. The virus binding and membrane fusion steps were unimpaired, but only very limited amounts of virus protein and RNA synthesized were detected after the infection. In a minigenome chloramphenicol acetyltransferase assay, MODE-K cells were as able as the permissive HeLa cells in supporting MV polymerase activity. The restriction phenotype of MODE-K cells could be alleviated by providing, in trans, either N-P-L or N-P functional protein complexes but not by P-L complexes or individual N, P, and L proteins. Several human x mouse (HeLa x MODE-K) somatic hybrid clones expressing human CD46 were isolated and found to be either nonpermissive or permissive according to their human chromosomal contents. The MV-restricted phenotype exhibited by the MODE-K cell line suggests that a cellular factor(s) can control MV transcription, possibly by stabilizing the incoming virus polymerase templates.

* Corresponding author. Mailing address: Immunité & Infections Virales, VPV, CNRS-UCBL UMR 5537, Faculté de Médecine Lyon RTH Laennec, 69372 Lyon Cedex 08, France. Phone: 33 4 78 77 86 18. Fax: 33 4 78 77 87 54. E-mail: gerlier{at}laennec.univ-lyon1.fr.

Journal of Virology, June 2002, p. 6121-6130, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6121-6130.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pohl, C., Duprex, W. P., Krohne, G., Rima, B. K., Schneider-Schaulies, S. (2007). Measles virus M and F proteins associate with detergent-resistant membrane fractions and promote formation of virus-like particles. J. Gen. Virol. 88: 1243-1250 [Abstract] [Full Text]  
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  • Chen, M., Cortay, J.-C., Logan, I. R., Sapountzi, V., Robson, C. N., Gerlier, D. (2005). Inhibition of Ubiquitination and Stabilization of Human Ubiquitin E3 Ligase PIRH2 by Measles Virus Phosphoprotein. J. Virol. 79: 11824-11836 [Abstract] [Full Text]  
  • Plumet, S., Duprex, W. P., Gerlier, D. (2005). Dynamics of Viral RNA Synthesis during Measles Virus Infection. J. Virol. 79: 6900-6908 [Abstract] [Full Text]  


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