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Journal of Virology, June 2002, p. 5925-5936, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.5925-5936.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Discordance between Frequency of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Gamma Interferon-Producing CD4+ T Cells and HIV-1-Specific Lymphoproliferation in HIV-1-Infected Subjects with Active Viral Replication

B. E. Palmer, E. Boritz, N. Blyveis, and C. C. Wilson*

Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262

Received 9 January 2002/ Accepted 11 March 2002

One hallmark of uncontrolled, chronic human immunodeficiency virus type 1 (HIV-1) infection is the absence of strong HIV-1-specific, CD4+ T-cell-proliferative responses, yet the mechanism underlying this T helper (Th)-cell defect remains controversial. To better understand the impact of HIV-1 replication on Th-cell function, we compared the frequency of CD4+ Th-cell responses based on production of gamma interferon to lymphoproliferative responses directed against HIV-1 proteins in HIV-1-infected subjects with active in vivo viral replication versus those on suppressed highly active antiretroviral therapy (HAART). No statistically significant differences in the frequencies of cytokine-secreting, HIV-1-specific CD4+ T cells between the donor groups were found, despite differences in viral load and treatment status. However, HIV-1-specific lymphoproliferative responses were significantly greater in the subjects with HAART suppression than in subjects with active viral replication. Similar levels of HIV-1 RNA were measured in T-cell cultures stimulated with HIV-1 antigens regardless of donor in vivo viral loads, but only HIV-1-specific CD4+ T cells from subjects with HAART suppression proliferated in vitro, suggesting that HIV-1 replication in vitro does not preclude HIV-1-specific lymphoproliferation. This study demonstrates a discordance between the frequency and proliferative capacity of HIV-1-specific CD4+ T cells in subjects with ongoing in vivo viral replication and suggests that in vivo HIV-1 replication contributes to the observed defect in HIV-1-specific CD4+ T-cell proliferation.


* Corresponding author. Mailing address: Divisions of Clinical Immunology and Infectious Diseases, University of Colorado Health Sciences Center, Campus Box B-164, 4200 E. Ninth Ave., Denver, CO 80262. Phone: (303) 315-6659. Fax: (303) 315-7642. E-mail: Cara.Wilson{at}UCHSC.edu.


Journal of Virology, June 2002, p. 5925-5936, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.5925-5936.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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