This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Palmer, B. E.
Right arrow Articles by Wilson, C. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palmer, B. E.
Right arrow Articles by Wilson, C. C.

 Previous Article  |  Next Article 

Journal of Virology, June 2002, p. 5925-5936, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.5925-5936.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Discordance between Frequency of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Gamma Interferon-Producing CD4+ T Cells and HIV-1-Specific Lymphoproliferation in HIV-1-Infected Subjects with Active Viral Replication

B. E. Palmer, E. Boritz, N. Blyveis, and C. C. Wilson*

Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262

Received 9 January 2002/ Accepted 11 March 2002

One hallmark of uncontrolled, chronic human immunodeficiency virus type 1 (HIV-1) infection is the absence of strong HIV-1-specific, CD4+ T-cell-proliferative responses, yet the mechanism underlying this T helper (Th)-cell defect remains controversial. To better understand the impact of HIV-1 replication on Th-cell function, we compared the frequency of CD4+ Th-cell responses based on production of gamma interferon to lymphoproliferative responses directed against HIV-1 proteins in HIV-1-infected subjects with active in vivo viral replication versus those on suppressed highly active antiretroviral therapy (HAART). No statistically significant differences in the frequencies of cytokine-secreting, HIV-1-specific CD4+ T cells between the donor groups were found, despite differences in viral load and treatment status. However, HIV-1-specific lymphoproliferative responses were significantly greater in the subjects with HAART suppression than in subjects with active viral replication. Similar levels of HIV-1 RNA were measured in T-cell cultures stimulated with HIV-1 antigens regardless of donor in vivo viral loads, but only HIV-1-specific CD4+ T cells from subjects with HAART suppression proliferated in vitro, suggesting that HIV-1 replication in vitro does not preclude HIV-1-specific lymphoproliferation. This study demonstrates a discordance between the frequency and proliferative capacity of HIV-1-specific CD4+ T cells in subjects with ongoing in vivo viral replication and suggests that in vivo HIV-1 replication contributes to the observed defect in HIV-1-specific CD4+ T-cell proliferation.

* Corresponding author. Mailing address: Divisions of Clinical Immunology and Infectious Diseases, University of Colorado Health Sciences Center, Campus Box B-164, 4200 E. Ninth Ave., Denver, CO 80262. Phone: (303) 315-6659. Fax: (303) 315-7642. E-mail: Cara.Wilson{at}UCHSC.edu.

Journal of Virology, June 2002, p. 5925-5936, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.5925-5936.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • D'Souza, M., Fontenot, A. P., Mack, D. G., Lozupone, C., Dillon, S., Meditz, A., Wilson, C. C., Connick, E., Palmer, B. E. (2007). Programmed Death 1 Expression on HIV-Specific CD4+ T Cells Is Driven by Viral Replication and Associated with T Cell Dysfunction. J. Immunol. 179: 1979-1987 [Abstract] [Full Text]  
  • Tilton, J. C., Luskin, M. R., Johnson, A. J., Manion, M., Hallahan, C. W., Metcalf, J. A., McLaughlin, M., Davey, R. T. Jr., Connors, M. (2007). Changes in Paracrine Interleukin-2 Requirement, CCR7 Expression, Frequency, and Cytokine Secretion of Human Immunodeficiency Virus-Specific CD4+ T Cells Are a Consequence of Antigen Load. J. Virol. 81: 2713-2725 [Abstract] [Full Text]  
  • Younes, S.-A., Trautmann, L., Yassine-Diab, B., Kalfayan, L. H., Kernaleguen, A.-E., Cameron, T. O., Boulassel, R., Stern, L. J., Routy, J.-P., Grossman, Z., Dumont, A. R., Sekaly, R.-P. (2007). The Duration of Exposure to HIV Modulates the Breadth and the Magnitude of HIV-Specific Memory CD4+ T Cells. J. Immunol. 178: 788-797 [Abstract] [Full Text]  
  • Tilton, J. C., Johnson, A. J., Luskin, M. R., Manion, M. M., Yang, J., Adelsberger, J. W., Lempicki, R. A., Hallahan, C. W., McLaughlin, M., Mican, J. M., Metcalf, J. A., Iyasere, C., Connors, M. (2006). Diminished Production of Monocyte Proinflammatory Cytokines during Human Immunodeficiency Virus Viremia Is Mediated by Type I Interferons. J. Virol. 80: 11486-11497 [Abstract] [Full Text]  
  • Gorse, G. J., Simionescu, R. E., Patel, G. B. (2006). Cellular Immune Responses in Asymptomatic Human Immunodeficiency Virus Type 1 (HIV-1) Infection and Effects of Vaccination with Recombinant Envelope Glycoprotein of HIV-1. CVI 13: 26-32 [Abstract] [Full Text]  
  • Palmer, B. E., Blyveis, N., Fontenot, A. P., Wilson, C. C. (2005). Functional and Phenotypic Characterization of CD57+CD4+ T Cells and Their Association with HIV-1-Induced T Cell Dysfunction. J. Immunol. 175: 8415-8423 [Abstract] [Full Text]  
  • Morris, E. C., Tsallios, A., Bendle, G. M., Xue, S.-a., Stauss, H. J. (2005). A critical role of T cell antigen receptor-transduced MHC class I-restricted helper T cells in tumor protection. Proc. Natl. Acad. Sci. USA 102: 7934-7939 [Abstract] [Full Text]  
  • Boritz, E., Palmer, B. E., Wilson, C. C. (2004). Human Immunodeficiency Virus Type 1 (HIV-1)-Specific CD4+ T Cells That Proliferate In Vitro Detected in Samples from Most Viremic Subjects and Inversely Associated with Plasma HIV-1 Levels. J. Virol. 78: 12638-12646 [Abstract] [Full Text]  
  • Bain, C., Parroche, P., Lavergne, J. P., Duverger, B., Vieux, C., Dubois, V., Komurian-Pradel, F., Trepo, C., Gebuhrer, L., Paranhos-Baccala, G., Penin, F., Inchauspe, G. (2004). Memory T-Cell-Mediated Immune Responses Specific to an Alternative Core Protein in Hepatitis C Virus Infection. J. Virol. 78: 10460-10469 [Abstract] [Full Text]  
  • Kaufmann, D. E., Bailey, P. M., Sidney, J., Wagner, B., Norris, P. J., Johnston, M. N., Cosimi, L. A., Addo, M. M., Lichterfeld, M., Altfeld, M., Frahm, N., Brander, C., Sette, A., Walker, B. D., Rosenberg, E. S. (2004). Comprehensive Analysis of Human Immunodeficiency Virus Type 1-Specific CD4 Responses Reveals Marked Immunodominance of gag and nef and the Presence of Broadly Recognized Peptides. J. Virol. 78: 4463-4477 [Abstract] [Full Text]  
  • Yue, F. Y., Kovacs, C. M., Dimayuga, R. C., Parks, P., Ostrowski, M. A. (2004). HIV-1-Specific Memory CD4+ T Cells Are Phenotypically Less Mature Than Cytomegalovirus-Specific Memory CD4+ T Cells. J. Immunol. 172: 2476-2486 [Abstract] [Full Text]  
  • Harari, A., Petitpierre, S., Vallelian, F., Pantaleo, G. (2004). Skewed representation of functionally distinct populations of virus-specific CD4 T cells in HIV-1-infected subjects with progressive disease: changes after antiretroviral therapy. Blood 103: 966-972 [Abstract] [Full Text]  
  • Day, C. L., Walker, B. D. (2003). Progress in Defining CD4 Helper Cell Responses in Chronic Viral Infections. JEM 198: 1773-1777 [Full Text]  
  • Younes, S.-A., Yassine-Diab, B., Dumont, A. R., Boulassel, M.-R., Grossman, Z., Routy, J.-P., Sekaly, R.-P. (2003). HIV-1 Viremia Prevents the Establishment of Interleukin 2-producing HIV-specific Memory CD4+ T Cells Endowed with Proliferative Capacity. JEM 198: 1909-1922 [Abstract] [Full Text]  
  • Feeney, M. E., Draenert, R., Roosevelt, K. A., Pelton, S. I., McIntosh, K., Burchett, S. K., Mao, C., Walker, B. D., Goulder, P. J. R. (2003). Reconstitution of Virus-Specific CD4 Proliferative Responses in Pediatric HIV-1 Infection. J. Immunol. 171: 6968-6975 [Abstract] [Full Text]  
  • Iyasere, C., Tilton, J. C., Johnson, A. J., Younes, S., Yassine-Diab, B., Sekaly, R.-P., Kwok, W. W., Migueles, S. A., Laborico, A. C., Shupert, W. L., Hallahan, C. W., Davey, R. T. Jr., Dybul, M., Vogel, S., Metcalf, J., Connors, M. (2003). Diminished Proliferation of Human Immunodeficiency Virus-Specific CD4+ T Cells Is Associated with Diminished Interleukin-2 (IL-2) Production and Is Recovered by Exogenous IL-2. J. Virol. 77: 10900-10909 [Abstract] [Full Text]  
  • Kolber, M. A., Saenz, M. O. (2003). Antigen-Presenting Cell Modulation Induces a Memory Response to p24 in Peripheral Blood Leukocytes from Human Immunodeficiency Virus-Infected Individuals. CVI 10: 757-763 [Abstract] [Full Text]  
  • Scott, Z. A., Beaumier, C. M., Sharkey, M., Stevenson, M., Luzuriaga, K. (2003). HIV-1 Replication Increases HIV-Specific CD4+ T Cell Frequencies but Limits Proliferative Capacity in Chronically Infected Children. J. Immunol. 170: 5786-5792 [Abstract] [Full Text]  
  • Kawamura, T., Gatanaga, H., Borris, D. L., Connors, M., Mitsuya, H., Blauvelt, A. (2003). Decreased Stimulation of CD4+ T Cell Proliferation and IL-2 Production by Highly Enriched Populations of HIV-Infected Dendritic Cells. J. Immunol. 170: 4260-4266 [Abstract] [Full Text]  
  • Boritz, E., Palmer, B. E., Livingston, B., Sette, A., Wilson, C. C. (2003). Diverse Repertoire of HIV-1 p24-Specific, IFN-{gamma}-Producing CD4+ T Cell Clones Following Immune Reconstitution on Highly Active Antiretroviral Therapy. J. Immunol. 170: 1106-1116 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»