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Journal of Virology, June 2002, p. 5822-5828, Vol. 76, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.11.5822-5828.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mutation Spectra of Herpes Simplex Virus Type 1 Thymidine Kinase Mutants

Qiaosheng Lu, Ying T. Hwang, and Charles B. C. Hwang*

Department of Microbiology and Immunology, Medical College, SUNY Upstate Medical University, Syracuse, New York 13210

Received 9 January 2002/ Accepted 5 March 2002

To examine whether the exonuclease activity intrinsic to the polymerase (Pol) of herpes simplex virus type 1 can influence the mutational spectra, we applied the denaturing gradient gel electrophoresis (DGGE) system combined with sequencing to characterize thymidine kinase mutants isolated from both the wild-type virus and a mutant deficient in exonuclease activity, Y7. Wild-type viruses produced predominately frameshift mutations (67%), whereas Y7 replicated a significantly lower proportion of frameshifts (21%; P < 0.005). Furthermore, the majority of substitutions were transitional changes in both groups, although they distributed differently. The implications of these findings are discussed.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College, SUNY Upstate Medical University, 750 E. Adams St., Syracuse, NY 13210. Phone: (315) 464-8739. Fax: (315) 464-7680. E-mail: hwangc{at}mail.upstate.edu.

Journal of Virology, June 2002, p. 5822-5828, Vol. 76, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.11.5822-5828.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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