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Journal of Virology, June 2002, p. 5737-5747, Vol. 76, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.11.5737-5747.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

RelB-p50 NF-{kappa}B Complexes Are Selectively Induced by Cytomegalovirus Immediate-Early Protein 1: Differential Regulation of Bcl-xL Promoter Activity by NF-{kappa}B Family Members

H. Y. Jiang, Constantinos Petrovas, and Gail E. Sonenshein*

Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118-2394

Received 12 October 2001/ Accepted 25 February 2002

The NF-{kappa}B/Rel family has been implicated in control of transcription of the Bcl-xL gene, a target which mediates cell survival signals. The cytomegalovirus (CMV) immediate-early protein 1 (IE1) was previously shown to induce NF-{kappa}B activity. Here, we report that in both vascular smooth muscle cells (SMCs) and NIH 3T3 cells, surprisingly, IE1 failed to induce Bcl-xL promoter activity, although it induced activity of E8-CAT, a reporter construct driven by two copies of the NF-{kappa}B element upstream of the c-myc promoter (upstream regulatory element [URE]). Thus, the subunit nature of the NF-{kappa}B/Rel factors induced by IE1 was examined using immunofluorescence and immunoblotting. IE1 was found to selectively induce nuclear RelB and p50 in SMCs and NIH 3T3 cells. An increase in RelB protein mediated by IE1 could, in part, be related to an increase in steady-state relB mRNA levels. Consistent with this subunit identification, IE1 was unable to induce E8-CAT activity in relB-/- murine embryonic fibroblast cells. In cotransfection analysis of SMCs and NIH 3T3 cells, RelB and p50 proteins failed to induce Bcl-xL promoter activity while inducing E8-CAT. Furthermore, the NF-{kappa}B element of the Bcl-xL promoter only weakly bound RelB-p50 complexes compared to the URE NF-{kappa}B element. Overall, these findings demonstrate in SMCs and NIH 3T3 cells that the CMV IE1 protein selectively induces RelB and p50, which fail to activate the Bcl-xL promoter, indicating a strong specificity of binding and activity for the RelB member of the NF-{kappa}B family. Furthermore, our results implicate RelB in CMV infection of cells such as vascular SMCs.

* Corresponding author. Mailing address: Department of Biochemistry, Boston University School of Medicine, 715 Albany St., Boston, MA 02118. Phone: (617) 638-4120. Fax: (617) 638-4252. E-mail: gsonensh{at}bu.edu.

Journal of Virology, June 2002, p. 5737-5747, Vol. 76, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.11.5737-5747.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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