This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zeenko, V. V.
Right arrow Articles by Hohn, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeenko, V. V.
Right arrow Articles by Hohn, T.

 Previous Article  |  Next Article 

Journal of Virology, June 2002, p. 5678-5691, Vol. 76, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.11.5678-5691.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Eukaryotic Elongation Factor 1A Interacts with the Upstream Pseudoknot Domain in the 3' Untranslated Region of Tobacco Mosaic Virus RNA

Vladimir V. Zeenko,1,{dagger} Lyubov A. Ryabova,1,{ddagger} Alexander S. Spirin,2 Helen M. Rothnie,1 Daniel Hess,1 Karen S. Browning,3 and Thomas Hohn1*

Friedrich Miescher Institute, CH-4002 Basel, Switzerland,1 Institute of Protein Research, Russian Academy of Science, Pushchino, Russia,2 Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 787123

Received 11 September 2001/ Accepted 1 March 2002

The genomic RNA of tobacco mosaic virus (TMV), like that of other positive-strand RNA viruses, acts as a template for both translation and replication. The highly structured 3' untranslated region (UTR) of TMV RNAs plays an important role in both processes; it is not polyadenylated but ends with a tRNA-like structure (TLS) preceded by a conserved upstream pseudoknot domain (UPD). The TLS of tobamoviral RNAs can be specifically aminoacylated and, in this state, can interact with eukaryotic elongation factor 1A (eEF1A)/GTP with high affinity. Using a UV cross-linking assay, we detected another specific binding site for eEF1A/GTP, within the UPDs of TMV and crucifer-infecting tobamovirus (crTMV), that does not require aminoacylation. A mutational analysis revealed that UPD pseudoknot conformation and some conserved primary sequence elements are required for this interaction. Its possible role in the regulation of tobamovirus gene expression and replication is discussed.

* Corresponding author. Mailing address: Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland. Phone: (41) 61 697 72 66. Fax: (41) 61 697 39 76. E-mail: thomas.hohn{at}fmi.ch.

{dagger} Present address: Department of Biochemistry, University of California, Riverside, CA 92521.

{ddagger} Present address: Protéus S.A., 30000 Nîmes, France.

Journal of Virology, June 2002, p. 5678-5691, Vol. 76, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.11.5678-5691.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cheng, C.-W., Hsiao, Y.-Y., Wu, H.-C., Chuang, C.-M., Chen, J.-S., Tsai, C.-H., Hsu, Y.-H., Wu, Y.-C., Lee, C.-C., Meng, M. (2009). Suppression of Bamboo Mosaic Virus Accumulation by a Putative Methyltransferase in Nicotiana benthamiana. J. Virol. 83: 5796-5805 [Abstract] [Full Text]  
  • Hammond, J. A., Rambo, R. P., Filbin, M. E., Kieft, J. S. (2009). Comparison and functional implications of the 3D architectures of viral tRNA-like structures. RNA 15: 294-307 [Abstract] [Full Text]  
  • Yan, G., You, B., Chen, S.-P., Liao, J. K., Sun, J. (2008). Tumor Necrosis Factor-{alpha} Downregulates Endothelial Nitric Oxide Synthase mRNA Stability via Translation Elongation Factor 1-{alpha} 1. Circ. Res. 103: 591-597 [Abstract] [Full Text]  
  • Song, B.-H., Yun, S.-I., Choi, Y.-J., Kim, J.-M., Lee, C.-H., Lee, Y.-M. (2008). A complex RNA motif defined by three discontinuous 5-nucleotide-long strands is essential for Flavivirus RNA replication. RNA 14: 1791-1813 [Abstract] [Full Text]  
  • Komoda, K., Mawatari, N., Hagiwara-Komoda, Y., Naito, S., Ishikawa, M. (2007). Identification of a Ribonucleoprotein Intermediate of Tomato Mosaic Virus RNA Replication Complex Formation. J. Virol. 81: 2584-2591 [Abstract] [Full Text]  
  • Lin, J.-W., Ding, M.-P., Hsu, Y.-H., Tsai, C.-H. (2007). Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus. Nucleic Acids Res 35: 424-432 [Abstract] [Full Text]  
  • Nishikiori, M., Dohi, K., Mori, M., Meshi, T., Naito, S., Ishikawa, M. (2006). Membrane-bound tomato mosaic virus replication proteins participate in RNA synthesis and are associated with host proteins in a pattern distinct from those that are not membrane bound.. J. Virol. 80: 8459-8468 [Abstract] [Full Text]  
  • Mizumoto, H., Iwakawa, H.-o., Kaido, M., Mise, K., Okuno, T. (2006). Cap-Independent Translation Mechanism of Red Clover Necrotic Mosaic Virus RNA2 Differs from That of RNA1 and Is Linked to RNA Replication.. J. Virol. 80: 3781-3791 [Abstract] [Full Text]  
  • Thivierge, K., Nicaise, V., Dufresne, P. J., Cotton, S., Laliberte, J.-F., Le Gall, O., Fortin, M. G. (2005). Plant Virus RNAs. Coordinated Recruitment of Conserved Host Functions by (+) ssRNA Viruses during Early Infection Events. Plant Physiol. 138: 1822-1827 [Abstract] [Full Text]  
  • Krab, I. M., Caldwell, C., Gallie, D. R., Bol, J. F. (2005). Coat protein enhances translational efficiency of Alfalfa mosaic virus RNAs and interacts with the eIF4G component of initiation factor eIF4F. J. Gen. Virol. 86: 1841-1849 [Abstract] [Full Text]  
  • Panavas, T., Serviene, E., Brasher, J., Nagy, P. D. (2005). Yeast genome-wide screen reveals dissimilar sets of host genes affecting replication of RNA viruses. Proc. Natl. Acad. Sci. USA 102: 7326-7331 [Abstract] [Full Text]  
  • Barends, S., Rudinger-Thirion, J., Florentz, C., Giege, R., Pleij, C. W. A., Kraal, B. (2004). tRNA-Like Structure Regulates Translation of Brome Mosaic Virus RNA. J. Virol. 78: 4003-4010 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»