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Journal of Virology, June 2002, p. 5548-5556, Vol. 76, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.11.5548-5556.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterizing and Mapping Porcine Endogenous Retroviruses in Westran Pigs

Jun-Heon Lee,1 Graham C. Webb,2 Richard D. M. Allen,3 and Chris Moran1*

Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, The University of Sydney, Sydney, New South Wales 2006,1 Department of Obstetrics and Gynaecology, The Queen Elizabeth Hospital, and Department of Animal Science, The University of Adelaide, Woodville, South Australia 5011,,2 National Pancreas Transplantation Unit, Westmead Hospital, Westmead, New South Wales 2146, Australia3

Received 31 October 2001/ Accepted 28 February 2002

Since porcine endogenous retroviruses (PERVs) can infect cultured human cells, they are a potential hazard to xenotransplantation. For this reason, endogenous retroviruses from the Westran (Westmead Hospital transplantation) inbred line of pigs were analyzed by using consensus primers for the type A and type B viruses to amplify 1.8-kb envelope gene fragments. After preliminary analysis with restriction enzymes KpnI and MboI, 31 clones were sequenced. Between types A and B, five recombinant clones were identified. Fifty-five percent of clones (17 of 31) had premature stop codons within the envelope protein-encoding region. Endogenous retroviruses in Westran pigs were physically mapped by fluorescence in situ hybridization (FISH) using PERV-A and PERV-B envelope clones as probes to identify at least 32 integration sites (19 PERV-A sites and 13 PERV-B sites). The chromosomal sites of integration in the Westran strain are quite different from those in the European Large White pig. The recombinant clones suggest that defective PERVs could become infective through recombination and further that PERVs might recombine with human endogenous retroviruses in xenotransplants.

* Corresponding author. Mailing address: Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, The University of Sydney, Sydney, NSW 2006, Australia. Phone: 61-2-9351-3553. Fax: 61-2-9351-2114. E-mail: Chris.Moran{at}vetsci.usyd.edu.au.

Journal of Virology, June 2002, p. 5548-5556, Vol. 76, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.11.5548-5556.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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