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Journal of Virology, May 2002, p. 5285-5290, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.5285-5290.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression of a Membrane-Anchored Glycoprotein, the Influenza Virus Hemagglutinin, by Dicistronic Replicons Derived from the Poliovirus Genome

Marco Vignuzzi, Sylvie Gerbaud, Sylvie van der Werf, and Nicolas Escriou*

Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 75724 Paris Cédex 15, France

Received 8 October 2001/ Accepted 13 February 2002

Mono- and dicistronic poliovirus replicons were constructed to express the influenza virus hemagglutinin, retaining its signal peptide and transmembrane region. Picornavirus genomes do not normally encode glycoproteins, and only the dicistronic replicon, in which the foreign and poliovirus sequences were separated by the encephalomyocarditis virus internal ribosomal entry site, replicated and expressed glycosylated hemagglutinin.

* Corresponding author. Mailing address: Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cédex 15, France. Phone: 33-1-40-61-36-01. Fax: 33-1-40-61-32-41. E-mail: escriou{at}pasteur.fr.

Journal of Virology, May 2002, p. 5285-5290, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.5285-5290.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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