This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Spender, L. C. Articles by Farrell, P. J. Search for Related Content PubMed PubMed Citation Articles by Spender, L. C. Articles by Farrell, P. J.

Expression of Transcription Factor AML-2 (RUNX3, CBF-3) Is Induced by Epstein-Barr Virus EBNA-2 and Correlates with the B-Cell Activation Phenotype

Lindsay C. Spender,¹ Georgina H. Cornish,^1, Alexandra Sullivan,¹ and Paul J. Farrell^1,2*

Ludwig Institute for Cancer Research,¹ Virology and Cell Biology Section, Imperial College Faculty of Medicine, London W2 1PG, United Kingdom²

Received 29 November 2001/ Accepted 4 February 2002

To identify cell proteins regulated by the Epstein-Barr virus (EBV) transcription factor EBNA-2, we analyzed a cell line with conditional EBNA-2 activity by using microarray expression profiling. This led to the identification of two novel target genes induced by EBNA-2. The first of these, interleukin-16, is an immunomodulatory cytokine involved in the regulation of CD4 T cells. The second, AML-2, is a member of the Runt domain family of transcription factors. Quiescent B cells initially expressed AML-1 but, 48 h after virus infection, the levels of AML-1 decreased dramatically, whereas the amount of AML-2 protein increased. Analysis of a panel of B-cell lines indicated that AML-2 expression is normally predominant in EBV latency III, whereas AML-1 is associated with EBV latency I or EBV-negative cells. The AML genes are the first example of cell transcription factors whose expression correlates with the latency I/III phenotype.

Present address: ICRF, London WC2A 3PX, United Kingdom.

This article has been cited by other articles:

• Maier, S., Staffler, G., Hartmann, A., Hock, J., Henning, K., Grabusic, K., Mailhammer, R., Hoffmann, R., Wilmanns, M., Lang, R., Mages, J., Kempkes, B. (2006). Cellular Target Genes of Epstein-Barr Virus Nuclear Antigen 2. J. Virol. 80: 9761-9771 [Abstract] [Full Text]  
• Spender, L. C., Lucchesi, W., Bodelon, G., Bilancio, A., Karstegl, C. E., Asano, T., Dittrich-Breiholz, O., Kracht, M., Vanhaesebroeck, B., Farrell, P. J. (2006). Cell target genes of Epstein-Barr virus transcription factor EBNA-2: induction of the p55{alpha} regulatory subunit of PI3-kinase and its role in survival of EREB2.5 cells.. J. Gen. Virol. 87: 2859-2867 [Abstract] [Full Text]  
• Mohan, J., Dement-Brown, J., Maier, S., Ise, T., Kempkes, B., Tolnay, M. (2006). Epstein-Barr virus nuclear antigen 2 induces FcRH5 expression through CBF1. Blood 107: 4433-4439 [Abstract] [Full Text]  
• Zhao, B., Maruo, S., Cooper, A., R. Chase, M., Johannsen, E., Kieff, E., Cahir-McFarland, E. (2006). RNAs induced by Epstein-Barr virus nuclear antigen 2 in lymphoblastoid cell lines. Proc. Natl. Acad. Sci. USA 103: 1900-1905 [Abstract] [Full Text]  
• Grueter, B., Petter, M., Egawa, T., Laule-Kilian, K., Aldrian, C. J., Wuerch, A., Ludwig, Y., Fukuyama, H., Wardemann, H., Waldschuetz, R., Moroy, T., Taniuchi, I., Steimle, V., Littman, D. R., Ehlers, M. (2005). Runx3 Regulates Integrin {alpha}E/CD103 and CD4 Expression during Development of CD4-/CD8+ T Cells. J. Immunol. 175: 1694-1705 [Abstract] [Full Text]  
• Maier, S., Santak, M., Mantik, A., Grabusic, K., Kremmer, E., Hammerschmidt, W., Kempkes, B. (2005). A Somatic Knockout of CBF1 in a Human B-Cell Line Reveals that Induction of CD21 and CCR7 by EBNA-2 Is Strictly CBF1 Dependent and that Downregulation of Immunoglobulin M Is Partially CBF1 Independent. J. Virol. 79: 8784-8792 [Abstract] [Full Text]  
• Peng, R., Moses, S. C., Tan, J., Kremmer, E., Ling, P. D. (2005). The Epstein-Barr Virus EBNA-LP Protein Preferentially Coactivates EBNA2-Mediated Stimulation of Latent Membrane Proteins Expressed from the Viral Divergent Promoter. J. Virol. 79: 4492-4505 [Abstract] [Full Text]  
• Kwiatkowski, B., Chen, S. Y. J., Schubach, W. H. (2004). CKII Site in Epstein-Barr Virus Nuclear Protein 2 Controls Binding to hSNF5/Ini1 and Is Important for Growth Transformation. J. Virol. 78: 6067-6072 [Abstract] [Full Text]  
• Gordadze, A. V., Onunwor, C. W., Peng, R., Poston, D., Kremmer, E., Ling, P. D. (2004). EBNA2 Amino Acids 3 to 30 Are Required for Induction of LMP-1 and Immortalization Maintenance. J. Virol. 78: 3919-3929 [Abstract] [Full Text]  
• Barth, S., Liss, M., Voss, M. D., Dobner, T., Fischer, U., Meister, G., Grasser, F. A. (2003). Epstein-Barr Virus Nuclear Antigen 2 Binds via Its Methylated Arginine-Glycine Repeat to the Survival Motor Neuron Protein. J. Virol. 77: 5008-5013 [Abstract] [Full Text]  
• van Rees, B. P., Caspers, E., zur Hausen, A., van den Brule, A., Drillenburg, P., Weterman, M. A. J., Offerhaus, G. J. A. (2002). Different Pattern of Allelic Loss in Epstein-Barr Virus-Positive Gastric Cancer with Emphasis on the p53 Tumor Suppressor Pathway. Am. J. Pathol. 161: 1207-1213 [Abstract] [Full Text]