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Journal of Virology, May 2002, p. 4855-4865, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.4855-4865.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Ebola Virus VP40 Drives the Formation of Virus-Like Filamentous Particles Along with GP

Takeshi Noda,1,2 Hiroshi Sagara,3 Emiko Suzuki,3 Ayato Takada,2 Hiroshi Kida,1 and Yoshihiro Kawaoka2,4*

Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818,1 Division of Virology, Department of Microbiology and Immunology,2 Fine Morphology Laboratory, Department of Basic Medical Science, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan,3 Department of Pathobiological Science, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 537064

Received 13 November 2001/ Accepted 12 February 2002

Using biochemical assays, it has been demonstrated that expression of Ebola virus VP40 alone in mammalian cells induced production of particles with a density similar to that of virions. To determine the morphological properties of these particles, cells expressing VP40 and the particles released from the cells were examined by electron microscopy. VP40 induced budding from the plasma membrane of filamentous particles, which differed in length but had uniform diameters of approximately 65 nm. When the Ebola virus glycoprotein (GP) responsible for receptor binding and membrane fusion was expressed in cells, we found pleomorphic particles budding from the plasma membrane. By contrast, when GP was coexpressed with VP40, GP was found on the filamentous particles induced by VP40. These results demonstrated the central role of VP40 in formation of the filamentous structure of Ebola virions and may suggest an interaction between VP40 and GP in morphogenesis.

* Corresponding author. Mailing address: Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Phone: 81-3-5449-5310. Fax: 81-3-5449-5408. E-mail: kawaoka{at}ims.u-tokyo.ac.jp

Journal of Virology, May 2002, p. 4855-4865, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.4855-4865.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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