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Journal of Virology, January 2002, p. 127-135, Vol. 76, No. 1
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.1.127-135.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Adenovirus Hexon Protein Is a Potent Adjuvant for Activation of a Cellular Immune Response

Valérie Molinier-Frenkel,1* Renée Lengagne,1 Florence Gaden,2 Saw-See Hong,2 Jeannine Choppin,1 Hanne Gahery-Ségard,1 Pierre Boulanger,2 and Jean-Gérard Guillet1

Laboratoire d’Immunologie des Pathologies Infectieuses et Tumorales, INSERM U445, Institut Cochin de Génétique Moléculaire, Hôpital Cochin, 75014 Paris,1 Laboratoire de Virologie et Pathogénèse Moléculaires, CNRS UMR 5537, Faculté de Médecine RTH Laennec, 69008 Lyon, France2

Received 19 July 2001/ Accepted 2 October 2001

The capacity of recombinant adenoviruses (rAd) to induce immunization against their transgene products has been well documented. In the present study, we evaluated the vaccinal adjuvant role of rAd independently of its vector function. BALB/c mice received one subcutaneous injection of a mixture of six lipopeptides (LP6) used as a model immunogen, along with AdE1° (109 particles), a first-generation rAd empty vector. Although coinjected with a suboptimal dose of lipopeptides, AdE1° significantly improved the effectiveness of the vaccination, even in the absence of booster immunization. In contrast to mice that received LP6 alone or LP6 plus a mock adjuvant, mice injected with AdE1° plus LP6 developed both a polyspecific T-helper type 1 response and an effector CD8 T-cell response specific to at least two class I-restricted epitopes. The helper response was still observed when immunization was performed using LP6 plus a mixture of soluble capsid components released from detergent-disrupted virions. When mice were immunized with LP6 and each individual capsid component, i.e., hexon, penton base, or fiber, the results obtained suggested that hexon protein was responsible for the adjuvant effect exerted by disrupted Ad particles on the helper response to the immunogen. Our results thus have some important implications not only in vaccinology but also for gene therapy using rAd vectors.

* Corresponding author. Mailing address: Laboratoire d’Immunologie des Pathologies Infectieuses et Tumorales, INSERM U445, Institut Cochin de Génétique Moléculaire, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014 Paris, France. Phone: 33-1-40-51-65-02. Fax: 33-1-40-51-65-35. E-mail: frenkel{at}cochin.inserm.fr.

Journal of Virology, January 2002, p. 127-135, Vol. 76, No. 1
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.1.127-135.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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