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Journal of Virology, May 2001, p. 4444-4447, Vol. 75, No. 9
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.9.4444-4447.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Selection of Apoptosis-Deficient Adenovirus E4orf4 Mutants in Saccharomyces cerevisiae

Rana Afifi, Rakefet Sharf, Ronit Shtrichman,dagger and Tamar Kleinberger*

Gonda Center of Molecular Microbiology, Bruce Rappaport Faculty of Medicine, Technion, Haifa 31096, Israel

Received 13 November 2000/Accepted 19 January 2001

Adenovirus E4orf4 protein has been shown to induce p53-independent, protein phosphatase 2A (PP2A)-dependent apoptosis in transformed cells. Furthermore, E4orf4 also induces toxicity in Saccharomyces cerevisiae in a PP2A-dependent manner (D. Kornitzer and T. Kleinberger, submitted for publication). In this work, we utilized yeast cells to select for nonapoptotic E4orf4 mutants which, in turn, were shown to possess a diminished ability to bind PP2A. The success of this selection system will provide additional apoptosis-relevant mutants for E4orf4 research and strongly supports the relevance of E4orf4-induced toxicity in S. cerevisiae to E4orf4-induced apoptosis in mammalian cells.

* Corresponding author. Mailing address: Gonda Center of Molecular Microbiology, Bruce Rappaport Faculty of Medicine, Technion, Haifa 31096, Israel. Phone: 972-4-829-5257. Fax: 972-4-829-5225. E-mail: tamark{at}tx.technion.ac.il.

dagger Present address: Molecular, Cellular and Developmental Biology Department, University of California---Santa Barbara, Santa Barbara, CA 93106.

Journal of Virology, May 2001, p. 4444-4447, Vol. 75, No. 9
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.9.4444-4447.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.