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Journal of Virology, May 2001, p. 4158-4164, Vol. 75, No. 9
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.9.4158-4164.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Role of the Cytoplasmic Domain of the
-Subunit
of Integrin
v
6 in Infection by Foot-and-Mouth Disease
Virus
Laura C.
Miller,1
Wendy
Blakemore,1
Dean
Sheppard,2
Amha
Atakilit,2
Andrew M. Q.
King,1 and
Terry
Jackson1,*
Pirbright Laboratory, Institute for Animal
Health, Pirbright, Surrey GU24 ONF, United
Kingdom,1 and Lung Biology Center,
Cardiovascular Research Institute, Department of Medicine,
University of California, San Francisco, California
94143-08542
Received 17 November 2000/Accepted 9 February 2001
Field isolates of foot-and-mouth disease virus (FMDV) are believed
to use RGD-dependent integrins as cellular receptors in vivo. Using
SW480 cell transfectants, we have recently established that one such
integrin,
v
6, functions as a receptor for FMDV. This integrin was
shown to function as a receptor for virus attachment. However, it was
not known if the
v
6 receptor itself participated in the events
that follow virus binding to the host cell. In the present study, we
investigated the effects of various deletion mutations in the
6
cytoplasmic domain on infection. Our results show that although loss of
the
6 cytoplasmic domain has little effect on virus binding, this
domain is essential for infection, indicating a critical role in
postattachment events. The importance of endosomal acidification in
v
6-mediated infection was confirmed by experiments showing that
infection could be blocked by concanamycin A, a specific inhibitor of
the vacuolar ATPase.
*
Corresponding author. Mailing address: Pirbright
Laboratory, Institute for Animal Health, Ash Rd., Pirbright, Surrey
GU24 ONF, United Kingdom. Phone: 44-1483-232441. Fax: 44-1483-237161. E-mail: terry.jackson{at}bbsrc.ac.uk.
Journal of Virology, May 2001, p. 4158-4164, Vol. 75, No. 9
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.9.4158-4164.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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