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Journal of Virology, April 2001, p. 4008-4013, Vol. 75, No. 8
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.8.4008-4013.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Herpesvirus Saimiri Open Reading Frame 50 (Rta) Protein
Reactivates the Lytic Replication Cycle in a Persistently Infected
A549 Cell Line
Delyth J.
Goodwin,1
Matthew S.
Walters,1
Peter G.
Smith,1
Mathias
Thurau,2
Helmut
Fickenscher,2 and
Adrian
Whitehouse1,*
Molecular Medicine Unit, St. James's
University Hospital, University of Leeds, Leeds LS9 7TF, United
Kingdom,1 and Institut für
Klinische und Molekulare Virologie der
Friedrich-Alexander-Universität Erlangen-Nürnberg,
D-91054 Erlangen, Germany2
Received 6 November 2000/Accepted 15 January 2001
Herpesviruses occur in two distinct forms of infection, lytic
replication and latent persistence. In this study, we investigated the
molecular mechanisms that govern the latent-lytic switch in the
prototype gamma-2 herpesvirus, herpesvirus saimiri (HVS). We utilized a
persistently HVS-infected A549 cell line, in which HVS DNA is stably
maintained as nonintegrated circular episomes, to assess the role of
the open reading frame 50 (ORF 50) (Rta) proteins in the latent-lytic
switch. Northern blot analysis and virus recovery assays determined
that the ORF 50a gene product, when expressed under the control of a
constitutively active promoter, was sufficient to reactivate the entire
lytic replication cycle, producing infectious virus particles.
Furthermore, although the ORF 50 proteins of HVS strains A11 and C488
are structurally divergent, they were both capable of inducing the
lytic replication cycle in this model of HVS latency.
*
Corresponding author. Mailing address: Molecular
Medicine Unit, St. James's University Hospital, University of Leeds,
Leeds LS9 7TF, United Kingdom. Phone: 44 (0) 113 2066328. Fax: 44 (0) 113 2444475. E-mail: a.whitehouse{at}leeds.ac.uk.
Journal of Virology, April 2001, p. 4008-4013, Vol. 75, No. 8
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.8.4008-4013.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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