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Journal of Virology, April 2001, p. 3988-3992, Vol. 75, No. 8
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.8.3988-3992.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Amino Acid Deletion at Codon 67 and Thr-to-Gly Change at Codon 69 of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Confer Novel Drug Resistance Profiles

Tomozumi Imamichi,1,* Michael A. Murphy,1 Hiromi Imamichi,1 and H. Clifford Lane2

Laboratory of Molecular Retrovirology, Clinical Services Program, Science Applications International Corporation---Frederick, National Cancer Institute---Frederick, Frederick,1 and Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda,2 Maryland

Received 26 October 2000/Accepted 19 January 2001

The potential roles of an amino acid deletion at codon 67 (Delta 67) and a Thr-to-Gly change at codon 69 (T69G) in the reverse transcriptase of human immunodeficiency virus (HIV) type 1 in drug sensitivity and relative replication fitness were studied. Our results suggest that the Delta 67 and T69G changes can be categorized as mutations associated with multidrug resistance. The combination of both mutations with an L74I change (Delta 67+T69G/L74I) leads to a novel 3'-azido-3'-deoxythymidine resistance motif and compensates for impaired HIV replication.

* Corresponding author. Mailing address: Laboratory of Molecular Retrovirology, Clinical Services Program, SAIC---Frederick, NCI---Frederick, Building 550, Room 126, P.O. Box B, Frederick, MD 21702-1201. Phone: (301) 846-5450. Fax: (301) 846-6762. E-mail: timamichi{at}nih.gov.

Journal of Virology, April 2001, p. 3988-3992, Vol. 75, No. 8
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.8.3988-3992.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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