This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thimme, R.
Right arrow Articles by Chisari, F. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thimme, R.
Right arrow Articles by Chisari, F. V.

 Previous Article  |  Next Article 

Journal of Virology, April 2001, p. 3984-3987, Vol. 75, No. 8
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.8.3984-3987.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Degenerate Immunogenicity of an HLA-A2-Restricted Hepatitis B Virus Nucleocapsid Cytotoxic T-Lymphocyte Epitope That Is Also Presented by HLA-B51†

Robert Thimme,1 Kyong-Mi Chang,1,2 Janell Pemberton,1 Alessandro Sette,3 and Francis V. Chisari1,*

Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 920371; Department of Medicine, University of Pennsylvania and Philadelphia Veterans Administration Medical Center, Philadelphia, Pennsylvania 191042; and Epimmune, Inc., La Jolla, California 921213

Received 30 November 2000/Accepted 16 January 2001

The recent identification of hepatitis B virus (HBV) epitopes restricted by multiple HLA alleles has greatly expanded the epitope repertoire available for T-cell-mediated therapeutic vaccine development. The HLA-B51-restricted peptide HBc19-27 is particularly interesting because it is located entirely within the HLA-A2-restricted HBc18-27 epitope. Here we show that HLA-B51-restricted cytotoxic T lymphocytes specific for HBc19-27 from a patient with acute HBV infection were also able to lyse HLA-B51-positive target cells pulsed with HBc18-27 and to produce gamma interferon when stimulated by that peptide, implying that HBc18-27 can be presented by HLA-B51 as well as by HLA-A2. These results demonstrate the concept of degenerate immunogenicity across HLA class supertype boundaries in a human viral disease setting. In addition, they could facilitate the development of an epitope-based therapeutic vaccine to terminate chronic HBV infection that could provide a broad and diverse population coverage with a single peptide.

* Corresponding author. Mailing address: Department of Molecular and Experimental Medicine, SBR-10, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-8228. Fax: (858) 784-2960. E-mail address: fchisari{at}scripps.edu.

dagger Manuscript no. 13467-MEM from the Scripps Research Institute.

Journal of Virology, April 2001, p. 3984-3987, Vol. 75, No. 8
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.8.3984-3987.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tan, A. T., Loggi, E., Boni, C., Chia, A., Gehring, A. J., Sastry, K. S. R., Goh, V., Fisicaro, P., Andreone, P., Brander, C., Lim, S. G., Ferrari, C., Bihl, F., Bertoletti, A. (2008). Host Ethnicity and Virus Genotype Shape the Hepatitis B Virus-Specific T-Cell Repertoire. J. Virol. 82: 10986-10997 [Abstract] [Full Text]  
  • Stoffels, R. J., Spencer, H. G. (2008). An Asymmetric Model of Heterozygote Advantage at Major Histocompatibility Complex Genes: Degenerate Pathogen Recognition and Intersection Advantage. Genetics 178: 1473-1489 [Abstract] [Full Text]  
  • Frank, I., Budde, C., Fiedler, M., Dahmen, U., Viazov, S., Lu, M., Dittmer, U., Roggendorf, M. (2007). Acute Resolving Woodchuck Hepatitis Virus (WHV) Infection Is Associated with a Strong Cytotoxic T-Lymphocyte Response to a Single WHV Core Peptide. J. Virol. 81: 7156-7163 [Abstract] [Full Text]  
  • Bertoletti, A., Gehring, A. J. (2006). The immune response during hepatitis B virus infection. J. Gen. Virol. 87: 1439-1449 [Abstract] [Full Text]  
  • Wieland, S. F., Chisari, F. V. (2005). Stealth and Cunning: Hepatitis B and Hepatitis C Viruses. J. Virol. 79: 9369-9380 [Full Text]  
  • Kumar, P., Sulochana, P., Nirmala, G., Haridattatreya, M., Satchidanandam, V. (2004). Conserved amino acids 193-324 of non-structural protein 3 are a dominant source of peptide determinants for CD4+ and CD8+ T cells in a healthy Japanese encephalitis virus-endemic cohort. J. Gen. Virol. 85: 1131-1143 [Abstract] [Full Text]  
  • Thimme, R., Wieland, S., Steiger, C., Ghrayeb, J., Reimann, K. A., Purcell, R. H., Chisari, F. V. (2002). CD8+ T Cells Mediate Viral Clearance and Disease Pathogenesis during Acute Hepatitis B Virus Infection. J. Virol. 77: 68-76 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»