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Journal of Virology, April 2001, p. 3164-3174, Vol. 75, No. 7
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.7.3164-3174.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of T-Cell Epitopes in Nonstructural Proteins of Foot-and-Mouth Disease Virus

Esther Blanco,1 Mercedes Garcia-Briones,1,2 Arantza Sanz-Parra,1 Paula Gomes,3 Eliandre De Oliveira,3 Mari Luz Valero,3 David Andreu,3 Victoria Ley,1 and Francisco Sobrino1,2,*

Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid,1 Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Cantoblanco, 28049 Madrid,2 and Departament de Química Orgànica, Universitat de Barcelona, 08028 Barcelona,3 Spain

Received 3 July 2000/Accepted 27 December 2000

Porcine T-cell recognition of foot-and-mouth disease virus (FMDV) nonstructural proteins (NSP) was tested using in vitro lymphoproliferative responses. Lymphocytes were obtained from outbred pigs experimentally infected with FMDV. Of the different NSP, polypeptides 3A, 3B, and 3C gave the highest stimulations in the in vitro assays. The use of overlapping synthetic peptides allowed the identification of amino acid regions within these proteins that were efficiently recognized by the lymphocytes. The sequences of some of these antigenic peptides were highly conserved among different FMDV serotypes. They elicited major histocompatibility complex-restricted responses with lymphocytes from pigs infected with either a type C virus or reinfected with a heterologous FMDV. A tandem peptide containing the T-cell peptide 3A[21-35] and the B-cell antigenic site VP1[137-156] also efficiently stimulated lymphocytes from infected animals in vitro. Furthermore, this tandem peptide elicited significant levels of serotype-specific antiviral activity, a result consistent with the induction of anti-FMDV antibodies. Thus, inclusion in the peptide formulation of a T-cell epitope derived from the NSP 3A possessing the capacity to induce T helper activity can allow cooperative induction of anti-FMDV antibodies by B cells.

* Corresponding author. Mailing address: CBMSO, Cantoblanco 28049, Madrid, Spain. Phone: 34-91-6202300. Fax: 34-91-6202247. E-mail: fsobrino{at}cnb.uam.es.

Journal of Virology, April 2001, p. 3164-3174, Vol. 75, No. 7
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.7.3164-3174.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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