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Journal of Virology, March 2001, p. 3034-3037, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.3034-3037.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Activation of NFAT-Dependent Gene Expression by Nef: Conservation among Divergent Nef Alleles, Dependence on SH3 Binding and Membrane Association, and Cooperation with Protein Kinase C-theta

Aki Manninen,1 Päivi Huotari,1 Marita Hiipakka,1 G. Herma Renkema,1 and Kalle Saksela1,2,*

Institute of Medical Technology, University of Tampere, FIN-33014 Tampere,1 and Department of Clinical Chemistry, Tampere University Hospital, FIN-33521 Tampere,2 Finland

Received 12 September 2000/Accepted 18 December 2000

Here we show that the potential to regulate NFAT is a conserved property of different Nef alleles and that Nef residues involved in membrane targeting and SH3 binding are critical for this function. Cotransfection of an activated protein kinase C-theta (PKC-theta ) with Nef implicated PKC-theta as a possible physiological cofactor of Nef in promoting NFAT-dependent gene expression and T-cell activation.

* Corresponding author. Mailing address: Institute of Medical Technology, University of Tampere, Finn-Medi II Building, Room 4-137, Lenkkeilijankatu 8, FIN-33520 Tampere, Finland. Phone: 358-3-2157029. Fax: 358-3-215 8597. E-mail: kalle.saksela{at}uta.fi.

Journal of Virology, March 2001, p. 3034-3037, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.3034-3037.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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