This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rayevskaya, M. V.
Right arrow Articles by Frankel, F. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rayevskaya, M. V.
Right arrow Articles by Frankel, F. R.

 Previous Article  |  Next Article 

Journal of Virology, March 2001, p. 2786-2791, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2786-2791.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Systemic Immunity and Mucosal Immunity Are Induced against Human Immunodeficiency Virus Gag Protein in Mice by a New Hyperattenuated Strain of Listeria monocytogenes

Marina V. Rayevskaya and Fred R. Frankel*

Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104

Received 18 September 2000/Accepted 14 December 2000

Vaccines designed to control chronic infections by intracellular agents such as human immunodeficiency virus type 1 (HIV-1) require the induction of cell-mediated immune responses to rid the host of pathogen-infected cells. Listeria monocytogenes has characteristics that make it an attractive vaccine vector for use against such infections. Here we show that parenteral immunization with a new highly attenuated strain of this organism provided complete protection against systemic and mucosal challenges with a recombinant vaccinia virus expressing HIV-1 gag. Immunization also generated a strong, long-term memory cytotoxic-T-lymphocyte (CTL) response in spleen, mesenteric lymph nodes, and Peyer's patches directed against the gag protein. Oral immunization with this attenuated strain also produced complete, long-lasting protection against the recombinant virus but only against mucosal virus challenge. Curiously, oral immunization was associated with a transient CTL response in the three lymphoid tissues examined.

* Corresponding author. Mailing address: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104. Phone: (215) 898-8730. Fax: (215) 898-9557. E-mail: frankelf{at}mail.med.upenn.edu.

Journal of Virology, March 2001, p. 2786-2791, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2786-2791.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Orr, M. T., Orgun, N. N., Wilson, C. B., Way, S. S. (2007). Cutting Edge: Recombinant Listeria monocytogenes Expressing a Single Immune-Dominant Peptide Confers Protective Immunity to Herpes Simplex Virus-1 Infection. J. Immunol. 178: 4731-4735 [Abstract] [Full Text]  
  • Li, Z., Zhao, X., Zhou, C., Gu, B., Frankel, F. R. (2006). A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes.. Microbiology 152: 3091-3102 [Abstract] [Full Text]  
  • Zhao, X., Zhang, M., Li, Z., Frankel, F. R. (2006). Vaginal Protection and Immunity after Oral Immunization of Mice with a Novel Vaccine Strain of Listeria monocytogenes Expressing Human Immunodeficiency Virus Type 1 gag.. J. Virol. 80: 8880-8890 [Abstract] [Full Text]  
  • Zhao, X., Li, Z., Gu, B., Frankel, F. R. (2005). Pathogenicity and Immunogenicity of a Vaccine Strain of Listeria monocytogenes That Relies on a Suicide Plasmid To Supply an Essential Gene Product. Infect. Immun. 73: 5789-5798 [Abstract] [Full Text]  
  • Li, Z., Zhao, X., Higgins, D. E., Frankel, F. R. (2005). Conditional Lethality Yields a New Vaccine Strain of Listeria monocytogenes for the Induction of Cell-Mediated Immunity. Infect. Immun. 73: 5065-5073 [Abstract] [Full Text]  
  • Craft, N., Bruhn, K. W., Nguyen, B. D., Prins, R., Lin, J. W., Liau, L. M., Miller, J. F. (2005). The TLR7 Agonist Imiquimod Enhances the Anti-Melanoma Effects of a Recombinant Listeria monocytogenes Vaccine. J. Immunol. 175: 1983-1990 [Abstract] [Full Text]  
  • Feng, H., Zhang, D., Palliser, D., Zhu, P., Cai, S., Schlesinger, A., Maliszewski, L., Lieberman, J. (2005). Listeria-Infected Myeloid Dendritic Cells Produce IFN-{beta}, Priming T Cell Activation. J. Immunol. 175: 421-432 [Abstract] [Full Text]  
  • Buonaguro, L., Visciano, M. L., Tornesello, M. L., Tagliamonte, M., Biryahwaho, B., Buonaguro, F. M. (2005). Induction of Systemic and Mucosal Cross-Clade Neutralizing Antibodies in BALB/c Mice Immunized with Human Immunodeficiency Virus Type 1 Clade A Virus-Like Particles Administered by Different Routes of Inoculation. J. Virol. 79: 7059-7067 [Abstract] [Full Text]  
  • Kotton, C. N., Hohmann, E. L. (2004). Enteric Pathogens as Vaccine Vectors for Foreign Antigen Delivery. Infect. Immun. 72: 5535-5547 [Full Text]  
  • Zhang, H., Fayad, R., Wang, X., Quinn, D., Qiao, L. (2004). Human Immunodeficiency Virus Type 1 Gag-Specific Mucosal Immunity after Oral Immunization with Papillomavirus Pseudoviruses Encoding Gag. J. Virol. 78: 10249-10257 [Abstract] [Full Text]  
  • Stevens, R., Howard, K. E., Nordone, S., Burkhard, M., Dean, G. A. (2004). Oral Immunization with Recombinant Listeria monocytogenes Controls Virus Load after Vaginal Challenge with Feline Immunodeficiency Virus. J. Virol. 78: 8210-8218 [Abstract] [Full Text]  
  • Garulli, B., Kawaoka, Y., Castrucci, M. R. (2004). Mucosal and Systemic Immune Responses to a Human Immunodeficiency Virus Type 1 Epitope Induced upon Vaginal Infection with a Recombinant Influenza A Virus. J. Virol. 78: 1020-1025 [Abstract] [Full Text]  
  • Sewell, D. A., Douven, D., Pan, Z.-K., Rodriguez, A., Paterson, Y. (2004). Regression of HPV-Positive Tumors Treated With a New Listeria monocytogenes Vaccine. Arch Otolaryngol Head Neck Surg 130: 92-97 [Abstract] [Full Text]  
  • Pinto, A. R., Fitzgerald, J. C., Giles-Davis, W., Gao, G. P., Wilson, J. M., Ertl, H. C. J. (2003). Induction of CD8+ T Cells to an HIV-1 Antigen through a Prime Boost Regimen with Heterologous E1-Deleted Adenoviral Vaccine Carriers. J. Immunol. 171: 6774-6779 [Abstract] [Full Text]  
  • Neuhaus, F. C., Baddiley, J. (2003). A Continuum of Anionic Charge: Structures and Functions of D-Alanyl-Teichoic Acids in Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 67: 686-723 [Abstract] [Full Text]  
  • Harvey, T. J., Anraku, I., Linedale, R., Harrich, D., Mackenzie, J., Suhrbier, A., Khromykh, A. A. (2003). Kunjin Virus Replicon Vectors for Human Immunodeficiency Virus Vaccine Development. J. Virol. 77: 7796-7803 [Abstract] [Full Text]  
  • Peters, C., Peng, X., Douven, D., Pan, Z.-K., Paterson, Y. (2003). The Induction of HIV Gag-Specific CD8+ T Cells in the Spleen and Gut-Associated Lymphoid Tissue by Parenteral or Mucosal Immunization with Recombinant Listeria monocytogenes HIV Gag. J. Immunol. 170: 5176-5187 [Abstract] [Full Text]  
  • Saklani-Jusforgues, H., Fontan, E., Soussi, N., Milon, G., Goossens, P. L. (2003). Enteral Immunization with Attenuated Recombinant Listeria monocytogenes as a Live Vaccine Vector: Organ-Dependent Dynamics of CD4 T Lymphocytes Reactive to a Leishmania major Tracer Epitope. Infect. Immun. 71: 1083-1090 [Abstract] [Full Text]  
  • Novitsky, V., Cao, H., Rybak, N., Gilbert, P., McLane, M. F., Gaolekwe, S., Peter, T., Thior, I., Ndung'u, T., Marlink, R., Lee, T. H., Essex, M. (2002). Magnitude and Frequency of Cytotoxic T-Lymphocyte Responses: Identification of Immunodominant Regions of Human Immunodeficiency Virus Type 1 Subtype C. J. Virol. 76: 10155-10168 [Abstract] [Full Text]  
  • Angelakopoulos, H., Loock, K., Sisul, D. M., Jensen, E. R., Miller, J. F., Hohmann, E. L. (2002). Safety and Shedding of an Attenuated Strain of Listeria monocytogenes with a Deletion of actA/plcB in Adult Volunteers: a Dose Escalation Study of Oral Inoculation. Infect. Immun. 70: 3592-3601 [Abstract] [Full Text]  
  • Rayevskaya, M., Kushnir, N., Frankel, F. R. (2002). Safety and Immunogenicity in Neonatal Mice of a Hyperattenuated Listeria Vaccine Directed against Human Immunodeficiency Virus. J. Virol. 76: 918-922 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»