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Journal of Virology, March 2001, p. 2786-2791, Vol. 75, No. 6
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.6.2786-2791.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Systemic Immunity and Mucosal Immunity Are Induced against Human
Immunodeficiency Virus Gag Protein in Mice by a New Hyperattenuated
Strain of Listeria monocytogenes
Marina V.
Rayevskaya and
Fred R.
Frankel*
Department of Microbiology, University of
Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Received 18 September 2000/Accepted 14 December 2000
Vaccines designed to control chronic infections by intracellular
agents such as human immunodeficiency virus type 1 (HIV-1) require the
induction of cell-mediated immune responses to rid the host of
pathogen-infected cells. Listeria monocytogenes has characteristics that make it an attractive vaccine vector for use
against such infections. Here we show that parenteral immunization with
a new highly attenuated strain of this organism provided complete
protection against systemic and mucosal challenges with a recombinant
vaccinia virus expressing HIV-1 gag. Immunization also
generated a strong, long-term memory cytotoxic-T-lymphocyte (CTL)
response in spleen, mesenteric lymph nodes, and Peyer's patches
directed against the gag protein. Oral immunization with this attenuated strain also produced complete, long-lasting protection against the recombinant virus but only against mucosal virus challenge. Curiously, oral immunization was associated with a transient CTL response in the three lymphoid tissues examined.
*
Corresponding author. Mailing address: Department of
Microbiology, University of Pennsylvania School of Medicine,
Philadelphia, PA 19104. Phone: (215) 898-8730. Fax: (215)
898-9557. E-mail: frankelf{at}mail.med.upenn.edu.
Journal of Virology, March 2001, p. 2786-2791, Vol. 75, No. 6
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.6.2786-2791.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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