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Journal of Virology, March 2001, p. 2653-2659, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2653-2659.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Ross River Virus Glycoprotein-Pseudotyped Retroviruses and Stable Cell Lines for Their Production

C. Matthew Sharkey, Cynthia L. North, Richard J. Kuhn, and David Avram Sanders*

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392

Received 31 August 2000/Accepted 11 December 2000

Pseudotyped retroviruses have important applications as vectors for gene transfer and gene therapy and as tools for the study of viral glycoprotein function. Recombinant Moloney murine leukemia virus (Mo-MuLV)-based retrovirus particles efficiently incorporate the glycoproteins of the alphavirus Ross River virus (RRV) and utilize them for entry into cells. Stable cell lines that produce the RRV glycoprotein-pseudotyped retroviruses for prolonged periods of time have been constructed. The pseudotyped viruses have a broadened host range, can be concentrated to high titer, and mediate stable transduction of genes into cells. The RRV glycoprotein-pseudotyped retroviruses and the cells that produce them have been employed to demonstrate that RRV glycoprotein-mediated viral entry occurs through endocytosis and that membrane fusion requires acidic pH. Alphavirus glycoprotein-pseudotyped retroviruses have significant advantages as reagents for the study of the biochemistry and prevention of alphavirus entry and as preferred vectors for stable gene transfer and gene therapy protocols.

* Corresponding author. Mailing address: Department of Biological Sciences, Purdue University, 1392 Lilly Hall, West Lafayette, IN 47907. Phone: (765) 494-6453. Fax: (765) 496-1189. E-mail: retrovir{at}bragg.bio.purdue.edu.

Journal of Virology, March 2001, p. 2653-2659, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2653-2659.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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