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Journal of Virology, March 2001, p. 2653-2659, Vol. 75, No. 6
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.6.2653-2659.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Ross River Virus Glycoprotein-Pseudotyped
Retroviruses and Stable Cell Lines for Their Production
C. Matthew
Sharkey,
Cynthia
L.
North,
Richard J.
Kuhn, and
David Avram
Sanders*
Department of Biological Sciences, Purdue
University, West Lafayette, Indiana 47907-1392
Received 31 August 2000/Accepted 11 December 2000
Pseudotyped retroviruses have important applications as vectors for
gene transfer and gene therapy and as tools for the study of viral
glycoprotein function. Recombinant Moloney murine leukemia virus (Mo-MuLV)-based retrovirus particles efficiently incorporate the
glycoproteins of the alphavirus Ross River virus (RRV) and utilize them for entry into cells. Stable cell lines that produce the
RRV glycoprotein-pseudotyped retroviruses for
prolonged periods of time have been constructed. The
pseudotyped viruses have a broadened host range, can be
concentrated to high titer, and mediate stable transduction of genes
into cells. The RRV glycoprotein-pseudotyped retroviruses and the cells that produce them have been employed to
demonstrate that RRV glycoprotein-mediated viral entry
occurs through endocytosis and that membrane fusion requires acidic pH. Alphavirus glycoprotein-pseudotyped retroviruses
have significant advantages as reagents for the study of the
biochemistry and prevention of alphavirus entry and as preferred
vectors for stable gene transfer and gene therapy protocols.
*
Corresponding author. Mailing address: Department of
Biological Sciences, Purdue University, 1392 Lilly Hall, West
Lafayette, IN 47907. Phone: (765) 494-6453. Fax: (765) 496-1189. E-mail: retrovir{at}bragg.bio.purdue.edu.
Journal of Virology, March 2001, p. 2653-2659, Vol. 75, No. 6
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.6.2653-2659.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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