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Journal of Virology, March 2001, p. 2584-2596, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2584-2596.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Protein-DNA Binding and CpG Methylation at Nucleotide Resolution of Latency-Associated Promoters Qp, Cp, and LMP1p of Epstein-Barr Virus

Daniel Salamon,1,2 Maria Takacs,3 Dorina Ujvari,1 Jörg Uhlig,4 Hans Wolf,4 Janos Minarovits,1,* and Hans Helmut Niller4,*

Microbiological Research Group, National Center for Epidemiology, H-1529 Budapest,1 Division of Virology, National Center for Epidemiology, H-1097 Budapest,3 and Second Department of Pathology, Faculty of Medicine, Semmelweis University, H-1091 Budapest,2 Hungary, and Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, D-93053 Regensburg, Germany4

Received 20 October 2000/Accepted 12 December 2000

Epstein-Barr viral (EBV) latency-associated promoters Qp, Cp, and LMP1p are crucial for the regulated expression of the EBNA and LMP transcripts in dependence of the latency type. By transient transfection and in vitro binding analyses, many promoter elements and transcription factors have previously been shown to be involved in the activities of these promoters. However, the latency promoters have only partially been examined at the nucleotide level in vivo. Therefore, we undertook a comprehensive analysis of in vivo protein binding and CpG methylation patterns at these promoters in five representative cell lines and correlated the results with the known in vitro binding data and activities of these promoters from previous transfection experiments. Promoter activity inversely correlated with the methylation state of promoters, although Qp was a remarkable exception. Novel protein binding data were obtained for all promoters. For Cp, binding correlated well with promoter activity; for LMP1p and Qp, binding patterns looked similar regardless of promoter activity.

* Corresponding author. Mailing address for J. Minarovits: Microbiological Research Group, National Center for Epidemiology, Pihenö út 1, 1522 Budapest, Hungary. Phone: 36 (1) 394-5044. Fax: 36 (1) 394-5409. E-mail: mini{at}microbi.hu. Mailing address for H. H. Niller: Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany. Phone: 49 (941) 944-6451. Fax: 49 (941) 944-6402. E-mail: Hans-Helmut.Niller{at}klinik.uni-regensburg.de.

Journal of Virology, March 2001, p. 2584-2596, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2584-2596.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.