This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salamon, D.
Right arrow Articles by Niller, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salamon, D.
Right arrow Articles by Niller, H. H.

 Previous Article  |  Next Article 

Journal of Virology, March 2001, p. 2584-2596, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2584-2596.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Protein-DNA Binding and CpG Methylation at Nucleotide Resolution of Latency-Associated Promoters Qp, Cp, and LMP1p of Epstein-Barr Virus

Daniel Salamon,1,2 Maria Takacs,3 Dorina Ujvari,1 Jörg Uhlig,4 Hans Wolf,4 Janos Minarovits,1,* and Hans Helmut Niller4,*

Microbiological Research Group, National Center for Epidemiology, H-1529 Budapest,1 Division of Virology, National Center for Epidemiology, H-1097 Budapest,3 and Second Department of Pathology, Faculty of Medicine, Semmelweis University, H-1091 Budapest,2 Hungary, and Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, D-93053 Regensburg, Germany4

Received 20 October 2000/Accepted 12 December 2000

Epstein-Barr viral (EBV) latency-associated promoters Qp, Cp, and LMP1p are crucial for the regulated expression of the EBNA and LMP transcripts in dependence of the latency type. By transient transfection and in vitro binding analyses, many promoter elements and transcription factors have previously been shown to be involved in the activities of these promoters. However, the latency promoters have only partially been examined at the nucleotide level in vivo. Therefore, we undertook a comprehensive analysis of in vivo protein binding and CpG methylation patterns at these promoters in five representative cell lines and correlated the results with the known in vitro binding data and activities of these promoters from previous transfection experiments. Promoter activity inversely correlated with the methylation state of promoters, although Qp was a remarkable exception. Novel protein binding data were obtained for all promoters. For Cp, binding correlated well with promoter activity; for LMP1p and Qp, binding patterns looked similar regardless of promoter activity.

* Corresponding author. Mailing address for J. Minarovits: Microbiological Research Group, National Center for Epidemiology, Pihenö út 1, 1522 Budapest, Hungary. Phone: 36 (1) 394-5044. Fax: 36 (1) 394-5409. E-mail: mini{at}microbi.hu. Mailing address for H. H. Niller: Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany. Phone: 49 (941) 944-6451. Fax: 49 (941) 944-6402. E-mail: Hans-Helmut.Niller{at}klinik.uni-regensburg.de.

Journal of Virology, March 2001, p. 2584-2596, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2584-2596.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Salamon, D., Banati, F., Koroknai, A., Ravasz, M., Szenthe, K., Bathori, Z., Bakos, A., Niller, H. H., Wolf, H., Minarovits, J. (2009). Binding of CCCTC-binding factor in vivo to the region located between Rep* and the C promoter of Epstein-Barr virus is unaffected by CpG methylation and does not correlate with Cp activity. J. Gen. Virol. 90: 1183-1189 [Abstract] [Full Text]  
  • Fejer, G., Koroknai, A., Banati, F., Gyory, I., Salamon, D., Wolf, H., Niller, H. H., Minarovits, J. (2008). Latency type-specific distribution of epigenetic marks at the alternative promoters Cp and Qp of Epstein-Barr virus. J. Gen. Virol. 89: 1364-1370 [Abstract] [Full Text]  
  • Gerle, B., Koroknai, A., Fejer, G., Bakos, A., Banati, F., Szenthe, K., Wolf, H., Niller, H. H., Minarovits, J., Salamon, D. (2007). Acetylated Histone H3 and H4 Mark the Upregulated LMP2A Promoter of Epstein-Barr Virus in Lymphoid Cells. J. Virol. 81: 13242-13247 [Abstract] [Full Text]  
  • Chau, C. M., Zhang, X.-Y., McMahon, S. B., Lieberman, P. M. (2006). Regulation of Epstein-Barr Virus Latency Type by the Chromatin Boundary Factor CTCF.. J. Virol. 80: 5723-5732 [Abstract] [Full Text]  
  • Chau, C. M., Lieberman, P. M. (2004). Dynamic Chromatin Boundaries Delineate a Latency Control Region of Epstein-Barr Virus. J. Virol. 78: 12308-12319 [Abstract] [Full Text]  
  • Chan, A. T.C., Tao, Q., Robertson, K. D., Flinn, I. W., Mann, R. B., Klencke, B., Kwan, W. H., Leung, T. W.-T., Johnson, P. J., Ambinder, R. F. (2004). Azacitidine Induces Demethylation of the Epstein-Barr Virus Genome in Tumors. JCO 22: 1373-1381 [Abstract] [Full Text]  
  • de Jesus, O., Smith, P. R., Spender, L. C., Elgueta Karstegl, C., Niller, H. H., Huang, D., Farrell, P. J. (2003). Updated Epstein-Barr virus (EBV) DNA sequence and analysis of a promoter for the BART (CST, BARF0) RNAs of EBV. J. Gen. Virol. 84: 1443-1450 [Abstract] [Full Text]  
  • Yoshioka, M., Kikuta, H., Ishiguro, N., Ma, X., Kobayashi, K. (2003). Unique Epstein-Barr virus (EBV) latent gene expression, EBNA promoter usage and EBNA promoter methylation status in chronic active EBV infection. J. Gen. Virol. 84: 1133-1140 [Abstract] [Full Text]  
  • Borestrom, C., Zetterberg, H., Liff, K., Rymo, L. (2002). Functional Interaction of Nuclear Factor Y and Sp1 Is Required for Activation of the Epstein-Barr Virus C Promoter. J. Virol. 77: 821-829 [Abstract] [Full Text]  
  • Niller, H. H., Salamon, D., Uhlig, J., Ranf, S., Granz, M., Schwarzmann, F., Wolf, H., Minarovits, J. (2002). Nucleoprotein Structure of Immediate-Early Promoters Zp and Rp and of oriLyt of Latent Epstein-Barr Virus Genomes. J. Virol. 76: 4113-4118 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»