This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, W.-F.
Right arrow Articles by Wu, T.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, W.-F.
Right arrow Articles by Wu, T.-C.

 Previous Article  |  Next Article 

Journal of Virology, March 2001, p. 2368-2376, Vol. 75, No. 5
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.5.2368-2376.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Enhancement of Sindbis Virus Self-Replicating RNA Vaccine Potency by Linkage of Herpes Simplex Virus Type 1 VP22 Protein to Antigen

Wen-Fang Cheng,1,2 Chien-Fu Hung,1 Chee-Yin Chai,1,dagger Keng-Fu Hsu,1,Dagger Liangmai He,1 Morris Ling,1 and T.-C. Wu1,3,4,5,*

Departments of Pathology,1 Obstetrics and Gynecology,5 Molecular Microbiology and Immunology,3 and Oncology,4 Johns Hopkins Medical Institutions, Baltimore, Maryland, and Department of Obstetrics and Gynecology, National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan2

Received 23 August 2000/Accepted 4 December 2000

Recently, self-replicating and self-limiting RNA vaccines (RNA replicons) have emerged as an important form of nucleic acid vaccines. Self-replicating RNA eventually causes lysis of transfected cells and does not raise the concern associated with naked DNA vaccines of integration into the host genome. This is particularly important for development of vaccines targeting proteins that are potentially oncogenic. However, the potency of RNA replicons is significantly limited by their lack of intrinsic ability to spread in vivo. The herpes simplex virus type 1 protein VP22 has demonstrated the remarkable property of intercellular transport and provides the opportunity to enhance RNA replicon vaccine potency. We therefore created a novel fusion of VP22 with a model tumor antigen, human papillomavirus type 16 E7, in a Sindbis virus RNA replicon vector. The linkage of VP22 with E7 resulted in a significant enhancement of E7-specific CD8+ T-cell activities in vaccinated mice and converted a less effective RNA replicon vaccine into one with significant potency against E7-expressing tumors. These results indicate that fusion of VP22 to an antigen gene may greatly enhance the potency of RNA replicon vaccines.

* Corresponding author. Mailing address: Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Room 659, 720 Rutland Ave., Baltimore, MD 21205. Phone: (410) 614-3899. Fax: (410) 614-3548. E-mail: wutc{at}jhmi.edu.

dagger Present address: Department of Pathology, School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Dagger Present address: Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, Taiwan.

Journal of Virology, March 2001, p. 2368-2376, Vol. 75, No. 5
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.5.2368-2376.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Liao, C.-W., Chen, C.-A., Lee, C.-N., Su, Y.-N., Chang, M.-C., Syu, M.-H., Hsieh, C.-Y., Cheng, W.-F. (2005). Fusion Protein Vaccine by Domains of Bacterial Exotoxin Linked with a Tumor Antigen Generates Potent Immunologic Responses and Antitumor Effects. Cancer Res. 65: 9089-9098 [Abstract] [Full Text]  
  • Kim, T. W., Hung, C.-F., Boyd, D., Juang, J., He, L., Kim, J. W., Hardwick, J. M., Wu, T.-C. (2003). Enhancing DNA Vaccine Potency by Combining a Strategy to Prolong Dendritic Cell Life with Intracellular Targeting Strategies. J. Immunol. 171: 2970-2976 [Abstract] [Full Text]  
  • Hung, C.-F., He, L., Juang, J., Lin, T.-J., Ling, M., Wu, T.-C. (2002). Improving DNA Vaccine Potency by Linking Marek's Disease Virus Type 1 VP22 to an Antigen. J. Virol. 76: 2676-2682 [Abstract] [Full Text]  
  • Cheng, W.-F., Hung, C.-F., Chai, C.-Y., Hsu, K.-F., He, L., Rice, C. M., Ling, M., Wu, T.-C. (2001). Enhancement of Sindbis Virus Self-Replicating RNA Vaccine Potency by Linkage of Mycobacterium tuberculosis Heat Shock Protein 70 Gene to an Antigen Gene. J. Immunol. 166: 6218-6226 [Abstract] [Full Text]  
  • Hung, C.-F., Cheng, W.-F., Chai, C.-Y., Hsu, K.-F., He, L., Ling, M., Wu, T.-C. (2001). Improving Vaccine Potency Through Intercellular Spreading and Enhanced MHC Class I Presentation of Antigen. J. Immunol. 166: 5733-5740 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»