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Journal of Virology, February 2001, p. 1594-1600, Vol. 75, No. 4
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.4.1594-1600.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

V Domain of Human SLAM (CDw150) Is Essential for Its Function as a Measles Virus Receptor

Nobuyuki Ono, Hironobu Tatsuo, Kotaro Tanaka, Hiroko Minagawa, and Yusuke Yanagi*

Department of Virology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan

Received 25 September 2000/Accepted 13 November 2000

Human signaling lymphocytic activation molecule (SLAM; also known as CDw150) has been shown to be a cellular receptor for measles virus (MV). Chinese hamster ovary cells transfected with a mouse SLAM cDNA were not susceptible to MV and the vesicular stomatitis virus pseudotype bearing MV envelope proteins alone, indicating that mouse SLAM cannot act as an MV receptor. To determine the functional domain of the receptor, we tested the abilities of several chimeric SLAM proteins to function as MV receptors. The ectodomain of SLAM comprises the two immunoglobulin superfamily domains (V and C2). Various chimeric transmembrane proteins possessing the V domain of human SLAM were able to act as MV receptors, whereas a chimera consisting of human SLAM containing the mouse V domain instead of the human V domain no longer acted as a receptor. To examine the interaction between SLAM and MV envelope proteins, recombinant soluble forms of SLAM were produced. The soluble molecules possessing the V domain of human SLAM were shown to bind to cells expressing the MV hemagglutinin (H) protein but not to cells expressing the MV fusion protein or irrelevant envelope proteins. These results indicate that the V domain of human SLAM is necessary and sufficient to interact with the MV H protein and allow MV entry.

* Corresponding author. Mailing address: Department of Virology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. Phone: 81-92-642-6135. Fax: 81-92-642-6140. E-mail: yyanagi{at}virology.med.kyushu-u.ac.jp.

Journal of Virology, February 2001, p. 1594-1600, Vol. 75, No. 4
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.4.1594-1600.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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