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Journal of Virology, February 2001, p. 1565-1570, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1565-1570.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Human Papillomavirus Infection Requires Cell Surface Heparan Sulfate

Tzenan Giroglou, Luise Florin, Frank Schäfer, Rolf E. Streeck, and Martin Sapp*

Institute for Medical Microbiology and Hygiene, University of Mainz, 55101 Mainz, Germany

Received 20 September 2000/Accepted 2 November 2000

Using pseudoinfection of cell lines, we demonstrate that cell surface heparan sulfate is required for infection by human papillomavirus type 16 (HPV-16) and HPV-33 pseudovirions. Pseudoinfection was inhibited by heparin but not dermatan or chondroitin sulfate, reduced by reducing the level of surface sulfation, and abolished by heparinase treatment. Carboxy-terminally deleted HPV-33 virus-like particles still bound efficiently to heparin. The kinetics of postattachment neutralization by antiserum or heparin indicated that pseudovirions were shifted on the cell surface from a heparin-sensitive into a heparin-resistant mode of binding, possibly involving a secondary receptor. Alpha-6 integrin is not a receptor for HPV-33 pseudoinfection.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Universität Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany. Phone: 49-6131-393-0211. Fax: 49-6131-393-2359. E-mail: sapp{at}mail.uni-mainz.de.

Journal of Virology, February 2001, p. 1565-1570, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1565-1570.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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