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Journal of Virology, February 2001, p. 1557-1560, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1557-1560.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Ten-Kilodalton Domain in Ty3 Gag3-Pol3p between PR and RT Is Dispensable for Ty3 Transposition

Jonathan A. Claypool,1 Harmit S. Malik,2 Thomas H. Eickbush,2 and Suzanne B. Sandmeyer1,3,*

Department of Microbiology and Molecular Genetics1 and Department of Biological Chemistry,3 University of California, Irvine, California 92697-1700, and Department of Biology, University of Rochester, Rochester, New York 146272

Received 14 February 2000/Accepted 20 October 2000

Ty3 is a gypsy-type, retrovirus-like element found in the budding yeast Saccharomyces cerevisiae. In cells overexpressing Ty3 under the GAL1 upstream activation sequence, Ty3 RNA, proteins, and DNA are made. Elucidation of the molecular masses and amino-terminal sequences of protease and reverse transcriptase indicated the existence of an additional intervening domain, designated J, in the Ty3 Gag3-Pol3p polyprotein. A region analogous to J can be found in many retrotransposable elements closely related to Ty3; however, J does not correspond to any of the highly conserved retroviral protein domains. Ty3 mutants deleted for the J-coding region showed moderately reduced transposition frequency but greatly reduced levels of Ty3 DNA. These results show that under galactose regulation, the Ty3 J domain is not absolutely essential.

* Corresponding author. Mailing address: Department of Biological Chemistry, Med. Sci. I D240, College of Medicine, University of California at Irvine, Irvine, CA 92697. Phone: (949) 824-7571. Fax: (949) 824-2688. E-mail: sbsandme{at}uci.edu.

Journal of Virology, February 2001, p. 1557-1560, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1557-1560.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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