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Journal of Virology, February 2001, p. 1516-1521, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1516-1521.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Assessment of Adenovirus Serum Neutralizing Antibody Titer Based on Quantitative, Morphometric Evaluation of Capsid Binding and Intracellular Trafficking: Population Analysis of Adenovirus Capsid Association with Cells Is Predictive of Adenovirus Infectivity

Theresa Vincent,1,2 Ben-Gary Harvey,1 Suzanne M. Hogan,1 Christopher J. Bailey,1,3 Ronald G. Crystal,1,4 and Philip L. Leopold1,*

Division of Pulmonary and Critical Care Medicine,1 Weill Graduate School of Medical Sciences,3 and Institute of Genetic Medicine,4 Weill Medical College of Cornell University, New York, New York, and the Karolinska Institute, Stockholm, Sweden2

Received 28 July 2000/Accepted 30 October 2000

Neutralizing antiviral antibodies are typically detected on the basis of inhibition of viral function, such as propagation of a viral infection or inhibition of viral gene expression. Evidence is presented that anti-adenovirus neutralizing antibodies can be evaluated by analysis of cell-associated capsids or by analysis of intracellular trafficking of the capsids within 1 h after infection. Quantitative analyses of these morphologic parameters represent rapid, broadly applicable, functional assays for the detection of anti-adenovirus neutralizing antibodies.

* Corresponding author. Mailing address: Weill Medical College of Cornell University, New York Presbyterian Hospital, 520 East 70th Street, Ste. 505, New York, NY 10021. Phone: (212) 746-2255. Fax: (212) 746-8383. E-mail: geneticmedicine{at}med.cornell.edu.

Journal of Virology, February 2001, p. 1516-1521, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1516-1521.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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