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Journal of Virology, February 2001, p. 1408-1413, Vol. 75, No. 3
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.3.1408-1413.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Identification of Two Prion Protein Regions That
Modify Scrapie Incubation Time
Surachai
Supattapone,1,2
Tamaki
Muramoto,2,
Giuseppe
Legname,1,2
Ingrid
Mehlhorn,2,
Fred E.
Cohen,1,3,4,5
Stephen J.
DeArmond,1,6
Stanley B.
Prusiner,1,2,5 and
Michael R.
Scott1,2,*
Institute for Neurodegenerative
Diseases1 and Departments of
Neurology,2
Medicine,3 Pharmaceutical
Chemistry,4 Biochemistry and
Biophysics,5 and
Pathology,6 University of California,
San Francisco, California 94143
Received 8 August 2000/Accepted 23 October 2000
A series of prion transmission experiments was performed in
transgenic (Tg) mice expressing either wild-type, chimeric, or truncated prion protein (PrP) molecules. Following inoculation with
Rocky Mountain Laboratory (RML) murine prions, scrapie incubation times
for Tg(MoPrP)4053, Tg(MHM2)294/Prnp0/0, and
Tg(MoPrP,
23-88)9949/Prnp0/0 mice were ~50, 120, and
160 days, respectively. Similar scrapie incubation times were obtained
after inoculation of these lines of Tg mice with either MHM2(MHM2(RML))
or MoPrP(
23-88)(RML) prions, excluding the possibility that
sequence-dependent transmission barriers could account for the observed
differences. Tg(MHM2)294/Prnp0/0 mice displayed prolonged
scrapie incubation times with four different strains of murine prions.
These data provide evidence that the N terminus of MoPrP and the
chimeric region of MHM2 PrP (residues 108 through 111) both influence
the inherent efficiency of prion propagation.
*
Corresponding author. Mailing address: Institute for
Neurodegenerative Diseases, Box 0518, University of California, San
Francisco, CA 94143-0518. Phone: (415) 476-4482. Fax: (415) 476-8386.

Present address: Department of Neurological Science, Tohoku
University School of Medicine, Aoba-ku, Sendai, Japan 980-8575.

Present address: Montclair Group, Tal Biosciences, Alameda, CA
94501.
Journal of Virology, February 2001, p. 1408-1413, Vol. 75, No. 3
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.3.1408-1413.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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