Identification of Two Prion Protein Regions That Modify Scrapie Incubation Time

doi:10.1128/JVI.75.3.1408-1413.2001

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Journal of Virology, February 2001, p. 1408-1413, Vol. 75, No. 3
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.3.1408-1413.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Two Prion Protein Regions That Modify Scrapie Incubation Time

Surachai Supattapone,¹^,² Tamaki Muramoto,²^, Giuseppe Legname,¹^,² Ingrid Mehlhorn,²^, Fred E. Cohen,¹^,³^,⁴^,⁵ Stephen J. DeArmond,¹^,⁶ Stanley B. Prusiner,¹^,²^,⁵ and Michael R. Scott¹^,²^,^*

Institute for Neurodegenerative Diseases¹ and Departments of Neurology,² Medicine,³ Pharmaceutical Chemistry,⁴ Biochemistry and Biophysics,⁵ and Pathology,⁶ University of California, San Francisco, California 94143

Received 8 August 2000/Accepted 23 October 2000

A series of prion transmission experiments was performed in transgenic (Tg) mice expressing either wild-type, chimeric, ortruncated prion protein (PrP) molecules. Following inoculationwith Rocky Mountain Laboratory (RML) murine prions, scrapie incubationtimes for Tg(MoPrP)4053, Tg(MHM2)294/Prnp^0/0, and Tg(MoPrP, $Delta$ 23-88)9949/Prnp^0/0 mice were ~50, 120, and 160 days, respectively. Similar scrapieincubation times were obtained after inoculation of these linesof Tg mice with either MHM2(MHM2(RML)) or MoPrP( $Delta$ 23-88)(RML) prions,excluding the possibility that sequence-dependent transmissionbarriers could account for the observed differences. Tg(MHM2)294/Prnp^0/0 mice displayed prolonged scrapie incubation times with four differentstrains of murine prions. These data provide evidence that theN terminus of MoPrP and the chimeric region of MHM2 PrP (residues108 through 111) both influence the inherent efficiency of prionpropagation.

^* Corresponding author. Mailing address: Institute for Neurodegenerative Diseases, Box 0518, University of California, San Francisco,CA 94143-0518. Phone: (415) 476-4482. Fax: (415) 476-8386.

Present address: Department of Neurological Science, Tohoku University School of Medicine, Aoba-ku, Sendai, Japan 980-8575.

Present address: Montclair Group, Tal Biosciences, Alameda, CA94501.

Journal of Virology, February 2001, p. 1408-1413, Vol. 75, No. 3
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.3.1408-1413.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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