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Journal of Virology, February 2001, p. 1274-1283, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1274-1283.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sorting of Marburg Virus Surface Protein and Virus Release Take Place at Opposite Surfaces of Infected Polarized Epithelial Cells

Christian Sänger,1 Elke Mühlberger,1 Elena Ryabchikova,2 Larissa Kolesnikova,1,2 Hans-Dieter Klenk,1 and Stephan Becker1,*

Institut für Virologie, Philipps-Universität Marburg, D-35037 Marburg, Germany,1 and State Research Center of Virology and Biotechnology "Vector" Institute of Molecular Biology, Laboratory of Ultrastructure and Pathomorphology, 633159 Koltsovo, Novosibirsk Region, Russia2

Received 2 August 2000/Accepted 25 October 2000

Marburg virus, a filovirus, causes severe hemorrhagic fever with hitherto poorly understood molecular pathogenesis. We have investigated here the vectorial transport of the surface protein GP of Marburg virus in polarized epithelial cells. To this end, we established an MDCKII cell line that was able to express GP permanently (MDCK-GP). The functional integrity of GP expressed in these cells was analyzed using vesicular stomatitis virus pseudotypes. Further experiments revealed that GP is transported in MDCK-GP cells mainly to the apical membrane and is released exclusively into the culture medium facing the apical membrane. When MDCKII cells were infected with Marburg virus, the majority of GP was also transported to the apical membrane, suggesting that the protein contains an autonomous apical transport signal. Release of infectious progeny virions, however, took place exclusively at the basolateral membrane of the cells. Thus, vectorial budding of Marburg virus is presumably determined by factors other than the surface protein.

* Corresponding author. Mailing address: Institut für Virologie der Philipps-Universität Marburg, Robert-Koch-Strasse 17, D-35037 Marburg, Germany. Phone: 49 (06421) 286-5433. Fax: 49 (06421) 286-5482. E-mail: becker{at}mailer.uni-marburg.de.

Journal of Virology, February 2001, p. 1274-1283, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1274-1283.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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