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Journal of Virology, February 2001, p. 1186-1194, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1186-1194.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genome Sequence of Bovine Herpesvirus 4, a Bovine Rhadinovirus, and Identification of an Origin of DNA Replication

Wolfgang Zimmermann,1,dagger Hermann Broll,2,Dagger Bernhard Ehlers,3 Hans-Jörg Buhk,2 André Rosenthal,1,§ and Michael Goltz*,3

Department of Genetic Analysis, Genome Sequencing Centre, Institut für Molekulare Biotechnologie, 07745 Jena,1 and Centre for Gene Technology2 and Project P24, Xenotransplantation,3 Robert Koch-Institut, 13353 Berlin, Germany

Received 25 August 2000/Accepted 7 November 2000

Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus of cattle. The complete long unique coding region (LUR) of BoHV-4 strain 66-p-347 was determined by a shotgun approach. Together with the previously published noncoding terminal repeats, the entire genome sequence of BoHV-4 is now available. The LUR consists of 108,873 bp with an overall G+C content of 41.4%. At least 79 open reading frames (ORFs) are present in this coding region, 17 of them unique to BoHV-4. In contrast to herpesvirus saimiri and human herpesvirus 8, BoHV-4 has a reduced set of ORFs homologous to cellular genes. Gene arrangement as well as phylogenetic analysis confirmed that BoHV-4 is a member of the genus Rhadinovirus. In addition, an origin of replication (ori) in the genome of BoHV-4 was identified by DpnI assays. A minimum of 1.69 kbp located between ORFs 69 and 71 was sufficient to act as a cis signal for replication.

* Corresponding author. Mailing address: Robert Koch-Institut, Nordufer 20, P24, 13353 Berlin, Germany. Phone: 49-1888-754-2271. Fax: 49-1888-754-2598 or 49-1888-754-2605. E-mail: goltzm{at}rki.de.

dagger Present address: Agowa GmbH, 12489 Berlin, Germany.

Dagger Present address: Department 213, Novel Foods and Genetic Engineering, Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, 14195 Berlin, Germany.

§ Present addresses: Friedrich Schiller Universität Jena, 0773 Jena, and metaGen GmbH, 14195 Berlin, Germany.

Journal of Virology, February 2001, p. 1186-1194, Vol. 75, No. 3
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.3.1186-1194.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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