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Journal of Virology, December 2001, p. 12431-12438, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12431-12438.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Glycoprotein K Specified by Herpes Simplex Virus Type 1 Is Expressed on Virions as a Golgi Complex-Dependent Glycosylated Species and Functions in Virion Entry

Timothy P. Foster, Galena V. Rybachuk, and Konstantin G. Kousoulas*

Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803

Received 3 July 2001/Accepted 5 September 2001

To facilitate detection of glycoprotein K (gK) specified by herpes simplex virus, a 12-amino-acid epitope tag was inserted within gK domain III. Recombinant virus gKprotC-DIII, expressing the tagged gK, was isolated. This virus formed wild-type plaques and replicated as efficiently as the wild-type KOS virus in Vero cells. Anti-protein C MAb detected high-mannose and Golgi complex-dependent glycosylated gK within cells as well as on purified virions. The gK-null virus Delta gK (gK-/-) entered Vero cells substantially more slowly than the wild-type KOS (gK+/+), while Delta gK virus grown in complementing VK302 cells (gK-/+) entered with entry kinetics similar to those of the KOS virus.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803. Phone: (225) 578-9682. Fax: (225) 578-9701. E-mail: vtgusk{at}lsu.edu.

Journal of Virology, December 2001, p. 12431-12438, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12431-12438.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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