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Journal of Virology, December 2001, p. 12331-12338, Vol. 75, No. 24
Texas Agricultural Experiment
Station,1 Department of
Entomology,2 and Department of
Biochemistry and Biophysics,3 Texas A&M
University, College Station, Texas 77843-2475
Received 12 June 2001/Accepted 19 September 2001
orf101 is a late gene of Autographa
californica nucleopolyhedrovirus (AcMNPV). It
encodes a protein of 42 kDa which is a component of the nucleocapsid of
budded virus (BV) and occlusion-derived virus (ODV). To reflect this
viral localization, the product of orf101 was named
BV/ODV-C42 (C42). C42 is predominantly detected within the
infected-cell nucleus: at 24 h postinfection (p.i.), it is
coincident with the virogenic stroma, but by 72 h p.i., the stroma
is minimally labeled while C42 is more uniformly located throughout the
nucleus. Yeast two-hybrid screens indicate that C42 is capable of
directly interacting with the viral proteins p78/83
(1629K) and ODV-EC27 (orf144). These
interactions were confirmed using blue native gels and Western blot
analyses. At 28 h p.i., C42 and p78/83 are detected in two
complexes: one at approximately 180 kDa and a
high-molecular-mass complex (500 to 600 kDa) which also contains EC27.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.24.12331-12338.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Identification of BV/ODV-C42, an Autographa
californica Nucleopolyhedrovirus orf101-Encoded
Structural Protein Detected in Infected-Cell Complexes with
ODV-EC27 and p78/83
*
Corresponding author. Mailing address: Department of
Entomology, Texas A&M University, College Station, TX 77843-2475. Phone: (979) 847-9036. Fax: (979) 845-8934. E-mail:
m-summers{at}tamu.edu.
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