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Journal of Virology, December 2001, p. 12331-12338, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12331-12338.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of BV/ODV-C42, an Autographa californica Nucleopolyhedrovirus orf101-Encoded Structural Protein Detected in Infected-Cell Complexes with ODV-EC27 and p78/83

Sharon C. Braunagel,1,2 Paula A. Guidry,1,2 German Rosas-Acosta,1,2 Luke Engelking,3 and Max D. Summers1,2,3,*

Texas Agricultural Experiment Station,1 Department of Entomology,2 and Department of Biochemistry and Biophysics,3 Texas A&M University, College Station, Texas 77843-2475

Received 12 June 2001/Accepted 19 September 2001

orf101 is a late gene of Autographa californica nucleopolyhedrovirus (AcMNPV). It encodes a protein of 42 kDa which is a component of the nucleocapsid of budded virus (BV) and occlusion-derived virus (ODV). To reflect this viral localization, the product of orf101 was named BV/ODV-C42 (C42). C42 is predominantly detected within the infected-cell nucleus: at 24 h postinfection (p.i.), it is coincident with the virogenic stroma, but by 72 h p.i., the stroma is minimally labeled while C42 is more uniformly located throughout the nucleus. Yeast two-hybrid screens indicate that C42 is capable of directly interacting with the viral proteins p78/83 (1629K) and ODV-EC27 (orf144). These interactions were confirmed using blue native gels and Western blot analyses. At 28 h p.i., C42 and p78/83 are detected in two complexes: one at approximately 180 kDa and a high-molecular-mass complex (500 to 600 kDa) which also contains EC27.

* Corresponding author. Mailing address: Department of Entomology, Texas A&M University, College Station, TX 77843-2475. Phone: (979) 847-9036. Fax: (979) 845-8934. E-mail: m-summers{at}tamu.edu.

Journal of Virology, December 2001, p. 12331-12338, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12331-12338.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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