This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tenllado, F.
Right arrow Articles by Díaz-Ruíz, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tenllado, F.
Right arrow Articles by Díaz-Ruíz, J. R.

 Previous Article  |  Next Article 

Journal of Virology, December 2001, p. 12288-12297, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12288-12297.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Double-Stranded RNA-Mediated Interference with Plant Virus Infection

F. Tenllado and J. R. Díaz-Ruíz*

Departamento de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain

Received 21 May 2001/Accepted 18 September 2001

Double-stranded RNA (dsRNA) has been shown to play a key role as an inducer of different interference phenomena occurring in both the plant and animal kingdoms. Here, we show that dsRNA derived from viral sequences can interfere with virus infection in a sequence-specific manner by directly delivering dsRNA to leaf cells either by mechanical inoculation or via an Agrobacterium-mediated transient-expression assay. We have successfully interfered with the infection of plants by three viruses belonging to the tobamovirus, potyvirus, and alfamovirus groups, demonstrating the reliability of the approach. We suggest that the effect mediated by dsRNA in plant virus infection resembles the analogous phenomenon of RNA interference observed in animals. The interference observed is sequence specific, is dose dependent, and is triggered by dsRNA but not single-stranded RNA. Our results support the view that a dsRNA intermediate in virus replication acts as efficient initiator of posttranscriptional gene silencing (PTGS) in natural virus infections, triggering the initiation step of PTGS that targets viral RNA for degradation.

* Corresponding author. Mailing address: Centro de Investigaciones Biológicas, Velázquez 144, 28006 Madrid, Spain. Phone: 34-9-1-5611800. Fax: 34-9-1-5627518. E-mail: jrdiazruiz{at}cib.csic.es.

Journal of Virology, December 2001, p. 12288-12297, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12288-12297.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Agrawal, N., Dasaradhi, P. V. N., Mohmmed, A., Malhotra, P., Bhatnagar, R. K., Mukherjee, S. K. (2003). RNA Interference: Biology, Mechanism, and Applications. Microbiol. Mol. Biol. Rev. 67: 657-685 [Abstract] [Full Text]  
  • Vanitharani, R., Chellappan, P., Fauquet, C. M. (2003). Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells. Proc. Natl. Acad. Sci. USA 100: 9632-9636 [Abstract] [Full Text]  
  • Adelman, Z. N., Sanchez-Vargas, I., Travanty, E. A., Carlson, J. O., Beaty, B. J., Blair, C. D., Olson, K. E. (2002). RNA Silencing of Dengue Virus Type 2 Replication in Transformed C6/36 Mosquito Cells Transcribing an Inverted-Repeat RNA Derived from the Virus Genome. J. Virol. 76: 12925-12933 [Abstract] [Full Text]  
  • Yelina, N. E., Savenkov, E. I., Solovyev, A. G., Morozov, S. Y., Valkonen, J. P. T. (2002). Long-Distance Movement, Virulence, and RNA Silencing Suppression Controlled by a Single Protein in Hordei- and Potyviruses: Complementary Functions between Virus Families. J. Virol. 76: 12981-12991 [Abstract] [Full Text]  
  • Coburn, G. A., Cullen, B. R. (2002). Potent and Specific Inhibition of Human Immunodeficiency Virus Type 1 Replication by RNA Interference. J. Virol. 76: 9225-9231 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»