This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herring, C.
Right arrow Articles by Scobie, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herring, C.
Right arrow Articles by Scobie, L.

 Previous Article  |  Next Article 

Journal of Virology, December 2001, p. 12252-12265, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12252-12265.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mapping Full-Length Porcine Endogenous Retroviruses in a Large White Pig

C. Herring,1 G. Quinn,1 R. Bower,1 N. Parsons,1 N. A. Logan,2 A. Brawley,2 K. Elsome,1 A. Whittam,1 X. M. Fernandez-Suarez,1 D. Cunningham,1 D. Onions,2 G. Langford,1 and L. Scobie2,*

Imutran Ltd. (a Novartis Pharma AG Company), Cambridge CB2 2YP,1 and Department of Veterinary Pathology, University of Glasgow, Glasgow G61 1QH,2 United Kingdom

Received 23 July 2001/Accepted 26 September 2001

Xenotransplantation may bridge the widening gap between the shortage of donor organs and the increasing number of patients waiting for transplantation. However, a major safety issue is the potential cross-species transmission of porcine endogenous retroviruses (PERV). This problem could be resolved if it is possible to produce pigs that do not contain replication-competent copies of this virus. In order to determine the feasibility of this, we have determined the number of potentially replication-competent full-length PERV proviruses and obtained data on their integration sites within the porcine genome. We have screened genomic DNA libraries from a Large White pig for potentially intact proviruses. We identified six unique PERV B proviruses that were apparently intact in all three genes, while the majority of isolated proviruses were defective in one or more genes. No intact PERV A proviruses were found in this pig, despite the identification of multiple defective A proviruses. Genotyping of 30 unrelated pigs for these unique proviruses showed a heterogeneous distribution. Two proviruses were uncommon, present in 7 of 30 and 3 of 30 pigs, while three were each present in 24 of 30 pigs, and one was present in 30 of 30 animals examined. Our data indicate that few PERV proviruses in Large White pigs are capable of productive infection and suggest that many could be removed by selective breeding. Further studies are required to determine if all potentially functional proviruses could be removed by breeding or whether gene knockout techniques will be required to remove the residuum.

* Corresponding author. Mailing address: Department of Veterinary Pathology, University of Glasgow, Switchback Rd., Garscube Estate, Glasgow G61 1QH, United Kingdom. Phone: 44 141 330 2283. Fax: 44 141 330 5602. E-mail: l.scobie{at}vet.gla.ac.uk.

Journal of Virology, December 2001, p. 12252-12265, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12252-12265.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Moalic, Y., Blanchard, Y., Felix, H., Jestin, A. (2006). Porcine Endogenous Retrovirus Integration Sites in the Human Genome: Features in Common with Those of Murine Leukemia Virus. J. Virol. 80: 10980-10988 [Abstract] [Full Text]  
  • Bartosch, B., Stefanidis, D., Myers, R., Weiss, R., Patience, C., Takeuchi, Y. (2004). Evidence and Consequence of Porcine Endogenous Retrovirus Recombination. J. Virol. 78: 13880-13890 [Abstract] [Full Text]  
  • Scobie, L., Taylor, S., Logan, N. A., Meikle, S., Onions, D., Patience, C., Quinn, G. (2004). Characterization of germline porcine endogenous retroviruses from Large White pig. J. Gen. Virol. 85: 2421-2428 [Abstract] [Full Text]  
  • Wood, J. C., Quinn, G., Suling, K. M., Oldmixon, B. A., Van Tine, B. A., Cina, R., Arn, S., Huang, C. A., Scobie, L., Onions, D. E., Sachs, D. H., Schuurman, H.-J., Fishman, J. A., Patience, C. (2004). Identification of Exogenous Forms of Human-Tropic Porcine Endogenous Retrovirus in Miniature Swine. J. Virol. 78: 2494-2501 [Abstract] [Full Text]  
  • Scobie, L., Taylor, S., Wood, J. C., Suling, K. M., Quinn, G., Meikle, S., Patience, C., Schuurman, H.-J., Onions, D. E. (2004). Absence of Replication-Competent Human-Tropic Porcine Endogenous Retroviruses in the Germ Line DNA of Inbred Miniature Swine. J. Virol. 78: 2502-2509 [Abstract] [Full Text]  
  • Quinn, G., Wood, J., Suling, K., Arn, S., Sachs, D. H., Schuurman, H.-J., Patience, C. (2004). Genotyping of Porcine Endogenous Retroviruses from a Family of Miniature Swine. J. Virol. 78: 314-319 [Abstract] [Full Text]  
  • Klymiuk, N., Muller, M., Brem, G., Aigner, B. (2003). Characterization of Endogenous Retroviruses in Sheep. J. Virol. 77: 11268-11273 [Abstract] [Full Text]  
  • Klymiuk, N., Muller, M., Brem, G., Aigner, B. (2003). Recombination analysis of human-tropic porcine endogenous retroviruses. J. Gen. Virol. 84: 2729-2734 [Abstract] [Full Text]  
  • Wilson, C. A., Laeeq, S., Ritzhaupt, A., Colon-Moran, W., Yoshimura, F. K. (2002). Sequence Analysis of Porcine Endogenous Retrovirus Long Terminal Repeats and Identification of Transcriptional Regulatory Regions. J. Virol. 77: 142-149 [Abstract] [Full Text]  
  • Klymiuk, N., Muller, M., Brem, G., Aigner, B. (2002). Characterization of Porcine Endogenous Retrovirus {gamma}pro-pol Nucleotide Sequences. J. Virol. 76: 11738-11743 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»