cis Expression of DC-SIGN Allows for More Efficient Entry of Human and Simian Immunodeficiency Viruses via CD4 and a Coreceptor

doi:10.1128/JVI.75.24.12028-12038.2001

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Journal of Virology, December 2001, p. 12028-12038, Vol. 75, No. 24
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.24.12028-12038.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

cis Expression of DC-SIGN Allows for More Efficient Entry of Human and Simian Immunodeficiency Viruses via CD4 and a Coreceptor

Benhur Lee,¹^,²^,^* George Leslie,³ Elizabeth Soilleux,⁴ Una O'Doherty,³ Sarah Baik,¹ Ernest Levroney,¹ Karen Flummerfelt,¹ William Swiggard,³ Nicholas Coleman,⁴ Michael Malim,³ and Robert W. Doms³^,^*

Department of Microbiology, Immunology & Molecular Genetics, UCLA School of Medicine¹ and UCLA AIDS Institute,² Los Angeles, California 90095; Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104³; and Medical Research Council Cancer Cell Unit, Hutchison/MRC Research Center, Cambridge CB2 2XZ, United Kingdom⁴

Received 24 July 2001/Accepted 18 September 2001

DC-SIGN is a C-type lectin expressed on dendritic cells and restricted macrophage populations in vivo that binds gp120 andacts in trans to enable efficient infection of T cells by humanimmunodeficiency virus type 1 (HIV-1). We report here that DC-SIGN,when expressed in cis with CD4 and coreceptors, allowed more efficientinfection by both HIV and simian immunodeficiency virus (SIV)strains, although the extent varied from 2- to 40-fold, dependingon the virus strain. Expression of DC-SIGN on target cells didnot alleviate the requirement for CD4 or coreceptor for viralentry. Stable expression of DC-SIGN on multiple lymphoid linesenabled more efficient entry and replication of R5X4 and X4 viruses.Thus, 10- and 100-fold less 89.6 (R5/X4) and NL4-3 (X4), respectively,were required to achieve productive replication in DC-SIGN-transducedJurkat cells when compared to the parental cell line. In addition,DC-SIGN expression on T-cell lines that express very low levelsof CCR5 enabled entry and replication of R5 viruses in a CCR5-dependentmanner, a property not exhibited by the parental cell lines. Therefore,DC-SIGN expression can boost virus infection in cis and can expandviral tropism without affecting coreceptor preference. In addition,coexpression of DC-SIGN enabled some viruses to use alternatecoreceptors like STRL33 to infect cells, whereas in its absence,infection was not observed. Immunohistochemical and confocal microscopydata indicated that DC-SIGN was coexpressed and colocalized withCD4 and CCR5 on alveolar macrophages, underscoring the physiologicalsignificance of these cis enhancementeffects.

^* Corresponding author. Mailing address for Benhur Lee: Department of Microbiology, Immunology & Molecular Genetics, UCLA, 3825Molecular Sciences Building, 609 Charles E. Young Dr. East, LosAngeles, CA 90095-1489. Phone: (310) 794-2132. Fax: (310) 267-2580.E-mail: benhurL{at}microbio.ucla.edu. Mailing address for RobertW. Doms: Department of Microbiology, University of Pennsylvania,225 Johnson Pavilion, 36th and Hamilton Walk, Philadelphia, PA19104. Phone: (215) 898-0890. Fax: (215) 898-9557. E-mail: doms{at}mail.med.upenn.edu.

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