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Journal of Virology, December 2001, p. 11902-11906, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11902-11906.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transduction of Cellular Sequence by a Human Immunodeficiency Virus Type 1-Derived Vector

Guoli Sun,1 Patrick K. O'Neil,2 Hong Yu,3 Yacov Ron,1 Bradley D. Preston,2 and Joseph P. Dougherty1,*

Department of Molecular Genetics & Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 088541; Eccles Institute of Human Genetics, University of Utah, Salt Lake City, Utah 841122; and Section of Immunobiology and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 065203

Received 27 June 2001/Accepted 20 August 2001

During studies examining the rate of human immunodeficiency virus type 1 (HIV-1) mutation in a single cycle of replication, the 5' long terminal repeat of one progeny provirus was found to contain an insertion of 147 bp including an entire tRNA<UP><SUB>3</SUB><SUP>Lys</SUP></UP> sequence as well as an additional 66 bp insertion of nonviral origin. Database searches revealed that 65 of 66 bp aligned with the human CpG island sequence found on chromosomes 6, 14, and 17. Therefore it seems probable that it is of human cellular sequence origin and was transduced by HIV-1. This is the first demonstration that HIV-1 can capture a cellular sequence. The site of integration of the parental provirus was mapped to chromosome 1p32.1. Sequence with homology to the transduced CpG island was not found on chromosome 1, suggesting that the transduced cellular sequence was not linked to the site of viral integration.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, 675 Hoes Ln., Piscataway, NJ 08854. Phone: (732) 235-4588. Fax: (732) 235-5223. E-mail: doughejp{at}umdnj.edu.

Journal of Virology, December 2001, p. 11902-11906, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11902-11906.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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