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Journal of Virology, December 2001, p. 11863-11867, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11863-11867.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Pattern of Tegument-Capsid Interaction in the Herpes Simplex Virus Type 1 Virion Is Not Influenced by the Small Hexon-Associated Protein VP26

Dong-Hua Chen,1 Joanita Jakana,1 David McNab,2 Joyce Mitchell,2 Z. Hong Zhou,3 Matthew Dougherty,1 Wah Chiu,1 and Frazer J. Rixon2,*

Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine,1 and Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School,3 Houston, Texas 77030, and MRC Virology Unit, Institute of Virology, Glasgow G11 5JR, United Kingdom2

Received 7 June 2001/Accepted 10 July 2001

Examination of the three-dimensional structure of intact herpes simplex virus type 1 (HSV-1) virions had revealed that the icosahedrally symmetrical interaction between the tegument and capsid involves the pentons but not the hexons (Z. H. Zhou, D. H. Chen, J. Jakana, F. J. Rixon, and W. Chiu, J. Virol. 73:3210-3218, 1999). To account for this, we postulated that the presence of the small capsid protein, VP26, on top of the hexons was masking potential binding sites and preventing tegument attachment. We have now tested this hypothesis by determining the structure of virions lacking VP26. Apart from the obvious absence of VP26 from the capsids, the structures of the VP26 minus and wild-type virions were essentially identical. Notably, they showed the same tegument attachment patterns, thereby demonstrating that VP26 is not responsible for the divergent tegument binding properties of pentons and hexons.

* Corresponding author. Mailing address: MRC Virology Unit, Institute of Virology, Church Street, Glasgow, United Kingdom, G11 5JR. Phone: 44141 330 4025. Fax: 44141 337 2236. E-mail: f.rixon{at}vir.gla.ac.uk.

Journal of Virology, December 2001, p. 11863-11867, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11863-11867.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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