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Journal of Virology, December 2001, p. 11755-11765, Vol. 75, No. 23
Institute for Animal Health, Pirbright
Laboratories, Woking, Surrey,1 School of
Biological Studies, University of Sussex, Falmer, Brighton,
Sussex,3 and School of Biochemistry and
Molecular Biology, University of Leeds, Leeds,
Yorkshire2 United Kingdom
Received 7 March 2001/Accepted 23 August 2001
The cellular secretory pathway is important during the assembly and
envelopment of viruses and also controls the transport of host
proteins, such as cytokines and major histocompatibility proteins, that
function during the elimination of viruses by the immune system.
African swine fever virus (ASFV) encodes at least 26 proteins with
stretches of hydrophobic amino acids suggesting entry into the
secretory pathway (R. J. Yanez, J. M. Rodriguez, M. L. Nogal, L. Yuste, C. Enriquez, J. F. Rodriguez, and E. Vinuela, Virology 208:249-278, 1995). To predict how and where
these potential membrane proteins function, we have studied the
integrity of the secretory pathway in cells infected with ASFV.
Remarkably, ASFV caused complete loss of immunofluorescence signal for
the trans Golgi network (TGN) marker protein TGN46 and
dispersed the AP1 TGN adapter complex. Loss of TGN46 signal was not due
to degradation of TGN46, suggesting redistribution of TGN46 to other
membrane compartments. ASFV markedly slowed transport of cathepsin D to lysosomes, demonstrating that loss of TGN structure correlated with
loss of TGN function. ASFV shows a tropism for macrophages, and it is
possible that ASFV compromises TGN function to augment the activity of
viral membrane proteins or to suppress the function of host
immunoregulatory proteins.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.23.11755-11765.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
The trans Golgi Network Is Lost from
Cells Infected with African Swine Fever Virus
*
Corresponding author. Mailing address: Institute for
Animal Health, Pirbright Laboratory, Ash Rd., Woking, Surrey GU240NF, United Kingdom. Phone: 44 01483 232441. Fax: 44 01483 232448. E-mail:
thomas.wileman{at}bbsrc.ac.uk.
Present address: Department of Cell Biology, Washington University
Medical School, St. Louis, MO 63110.
Journal of Virology, December 2001, p. 11755-11765, Vol. 75, No. 23
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.23.11755-11765.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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