This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thiebot, H.
Right arrow Articles by Le Grand, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thiebot, H.
Right arrow Articles by Le Grand, R.

 Previous Article  |  Next Article 

Journal of Virology, December 2001, p. 11594-11602, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11594-11602.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Early and Persistent Bone Marrow Hematopoiesis Defect in Simian/Human Immunodeficiency Virus-Infected Macaques despite Efficient Reduction of Viremia by Highly Active Antiretroviral Therapy during Primary Infection

Hugues Thiebot,1 Fawzia Louache,2 Bruno Vaslin,1 Thierry de Revel,1,3 Olivier Neildez,1 Jérome Larghero,1 William Vainchenker,2 Dominique Dormont,1 and Roger Le Grand1,*

CEA, Service de Neurovirologie, CRSSA, Ecole Pratique des Hautes Etudes, Institut Paris-Sud sur les Cytokines, 92 265 Fontenay aux Roses Cedex,1 Unité de Recherche en Hématologie et Cellules Souches, INERM 392, Institut Gustave Roussy, 94 805 Villejuif,2 and Hopital d'Instruction des Armées Percy, 92 141 Clamart Cedex,3 France

Received 30 May 2001/Accepted 30 August 2001

The hematological abnormalities observed in human immunodeficiency virus (HIV)-infected patients appear to be mainly due to bone marrow dysfunction. A macaque models of AIDS could greatly facilitate an in vivo approach to the pathogenesis of such dysfunction. Here, we evaluated in this model the impact of infection with a pathogenic simian/human immunodeficiency virus (SHIV) on bone marrow hematopoiesis. Three groups of macaques were inoculated with 50 50% median infective doses of pathogenic SHIV 89.P, which expresses env of dual-tropic HIV type 1 (HIV-1) 89.6 primary isolate. During the primary phase of infection, animals were treated with either a placebo or highly active antiretroviral therapy (HAART) combining zidovudine, lamivudine, and indinavir, initiated 4 or 72 h postinfection (p.i.) and administered twice a day until day 28 p.i. In both placebo-treated and HAART-treated animals, bone marrow colony-forming cells (CFC) progressively decreased quite early, during the first month p.i. One year p.i., both placebo- and HAART-treated animals displayed decreases in CFC to about 56% of preinfection values. At the same time, a dramatic decrease (greater than 77%) of bone marrow CD34+ long-term culture-initiating cells was noted in all animals were found. No statistically significant differences between placebo- and HAART-treated monkeys were found. These data argue for an early and profound alteration of myelopoiesis at the level of the most primitive CD34+ progenitor cells during SHIV infection, independently of the level of viremia, circulating CD4+ cell counts, or antiviral treatment.

* Corresponding author. Mailing address: Service de Neurovirologie, Commissariat à l'Energie Atomique, DSV/DRM/CRSSA, 60-68 Ave. de la Division Leclerc, B.P. 6, 92265 Fontenay-aux-Roses Cedex, France. Phone: 33 1 46 54 73 74. Fax: 33 1 46 54 77 26. E-mail: legrand{at}dsvidf.cea.fr.

Journal of Virology, December 2001, p. 11594-11602, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11594-11602.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Reeves, R. K., Fultz, P. N. (2008). Characterization of Plasmacytoid Dendritic Cells in Bone Marrow of Pig-Tailed Macaques. CVI 15: 35-41 [Abstract] [Full Text]  
  • Watanabe, S., Terashima, K., Ohta, S., Horibata, S., Yajima, M., Shiozawa, Y., Dewan, M. Z., Yu, Z., Ito, M., Morio, T., Shimizu, N., Honda, M., Yamamoto, N. (2007). Hematopoietic stem cell-engrafted NOD/SCID/IL2R{gamma}null mice develop human lymphoid systems and induce long-lasting HIV-1 infection with specific humoral immune responses. Blood 109: 212-218 [Abstract] [Full Text]  
  • Hosmalin, A., Lebon, P. (2006). Type I interferon production in HIV-infected patients. J. Leukoc. Biol. 80: 984-993 [Abstract] [Full Text]  
  • Williams, C. A., Mondal, D., Agrawal, K. C. (2005). The HIV-1 Tat Protein Enhances Megakaryocytic Commitment of K562 Cells by Facilitating CREB Transcription Factor Coactivation by CBP. Exp. Biol. Med. 230: 872-884 [Abstract] [Full Text]  
  • Thiebot, H., Vaslin, B., Derdouch, S., Bertho, J.-M., Mouthon, F., Prost, S., Gras, G., Ducouret, P., Dormont, D., Le Grand, R. (2005). Impact of bone marrow hematopoiesis failure on T-cell generation during pathogenic simian immunodeficiency virus infection in macaques. Blood 105: 2403-2409 [Abstract] [Full Text]  
  • Yamakami, K., Honda, M., Takei, M., Ami, Y., Kitamura, N., Nishinarita, S., Sawada, S., Horie, T. (2004). Early Bone Marrow Hematopoietic Defect in Simian/Human Immunodeficiency Virus C2/1-Infected Macaques and Relevance to Advance of Disease. J. Virol. 78: 10906-10910 [Abstract] [Full Text]  
  • Benlhassan-Chahour, K., Penit, C., Dioszeghy, V., Vasseur, F., Janvier, G., Riviere, Y., Dereuddre-Bosquet, N., Dormont, D., Le Grand, R., Vaslin, B. (2003). Kinetics of Lymphocyte Proliferation during Primary Immune Response in Macaques Infected with Pathogenic Simian Immunodeficiency Virus SIVmac251: Preliminary Report of the Effect of Early Antiviral Therapy. J. Virol. 77: 12479-12493 [Abstract] [Full Text]  
  • Giuffre, A. C., Higgins, J., Buckheit, R. W. Jr., North, T. W. (2003). Susceptibilities of Simian Immunodeficiency Virus to Protease Inhibitors. Antimicrob. Agents Chemother. 47: 1756-1759 [Abstract] [Full Text]  
  • Sandrin, V., Boson, B., Salmon, P., Gay, W., Negre, D., Le Grand, R., Trono, D., Cosset, F.-L. (2002). Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates. Blood 100: 823-832 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»