This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ghose, R.
Right arrow Articles by Rice, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ghose, R.
Right arrow Articles by Rice, A. P.

 Previous Article  |  Next Article 

Journal of Virology, December 2001, p. 11336-11343, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11336-11343.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Induction of TAK (Cyclin T1/P-TEFb) in Purified Resting CD4+ T Lymphocytes by Combination of Cytokines

Romi Ghose,dagger Li-Ying Liou, Christine H. Herrmann, and Andrew P. Rice*

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030

Received 16 May 2001/Accepted 3 August 2001

Combinations of cytokines are known to reactivate transcription and replication of latent human immunodeficiency virus type 1 (HIV-1) proviruses in resting CD4+ T lymphocytes isolated from infected individuals. Transcription of the HIV-1 provirus by RNA polymerase II is strongly stimulated by the viral Tat protein. Tat function is mediated by a cellular protein kinase known as TAK (cyclin T1/P-TEFb) that is composed of Cdk9 and cyclin T1. We have found that treatment of peripheral blood lymphocytes and purified resting CD4+ T lymphocytes with the combination of interleukin-2 (IL-2), IL-6, and tumor necrosis factor alpha resulted in an increase in Cdk9 and cyclin T1 protein levels and an increase in TAK enzymatic activity. The cytokine induction of TAK in resting CD4+ T lymphocytes did not appear to require proliferation of lymphocytes. These results suggest that induction of TAK by cytokines secreted in the microenvironment of lymphoid tissue may be involved in the reactivation of HIV-1 in CD4+ T lymphocytes harboring a latent provirus.

* Corresponding author. Mailing address: Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-5774. Fax: (713) 798-3490. E-mail: arice{at}bcm.tmc.edu.

dagger Present address: Amphioxus Cell Technologies, Houston, TX 77082.

Journal of Virology, December 2001, p. 11336-11343, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11336-11343.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pearson, R., Kim, Y. K., Hokello, J., Lassen, K., Friedman, J., Tyagi, M., Karn, J. (2008). Epigenetic Silencing of Human Immunodeficiency Virus (HIV) Transcription by Formation of Restrictive Chromatin Structures at the Viral Long Terminal Repeat Drives the Progressive Entry of HIV into Latency. J. Virol. 82: 12291-12303 [Abstract] [Full Text]  
  • Wang, Y., Dow, E. C., Liang, Y.-Y., Ramakrishnan, R., Liu, H., Sung, T.-L., Lin, X., Rice, A. P. (2008). Phosphatase PPM1A Regulates Phosphorylation of Thr-186 in the Cdk9 T-loop. J. Biol. Chem. 283: 33578-33584 [Abstract] [Full Text]  
  • Hou, T., Ray, S., Brasier, A. R. (2007). The Functional Role of an Interleukin 6-inducible CDK9{middle dot}STAT3 Complex in Human {gamma}-Fibrinogen Gene Expression. J. Biol. Chem. 282: 37091-37102 [Abstract] [Full Text]  
  • Berro, R., Kehn, K., de la Fuente, C., Pumfery, A., Adair, R., Wade, J., Colberg-Poley, A. M., Hiscott, J., Kashanchi, F. (2006). Acetylated Tat Regulates Human Immunodeficiency Virus Type 1 Splicing through Its Interaction with the Splicing Regulator p32.. J. Virol. 80: 3189-3204 [Abstract] [Full Text]  
  • Marshall, R. M., Salerno, D., Garriga, J., Grana, X. (2005). Cyclin T1 Expression Is Regulated by Multiple Signaling Pathways and Mechanisms during Activation of Human Peripheral Blood Lymphocytes. J. Immunol. 175: 6402-6411 [Abstract] [Full Text]  
  • Agbottah, E., de La Fuente, C., Nekhai, S., Barnett, A., Gianella-Borradori, A., Pumfery, A., Kashanchi, F. (2005). Antiviral Activity of CYC202 in HIV-1-infected Cells. J. Biol. Chem. 280: 3029-3042 [Abstract] [Full Text]  
  • Shan, B., Zhuo, Y., Chin, D., Morris, C. A., Morris, G. F., Lasky, J. A. (2005). Cyclin-dependent Kinase 9 Is Required for Tumor Necrosis Factor-{alpha}-stimulated Matrix Metalloproteinase-9 Expression in Human Lung Adenocarcinoma Cells. J. Biol. Chem. 280: 1103-1111 [Abstract] [Full Text]  
  • Han, Y., Lassen, K., Monie, D., Sedaghat, A. R., Shimoji, S., Liu, X., Pierson, T. C., Margolick, J. B., Siliciano, R. F., Siliciano, J. D. (2004). Resting CD4+ T Cells from Human Immunodeficiency Virus Type 1 (HIV-1)-Infected Individuals Carry Integrated HIV-1 Genomes within Actively Transcribed Host Genes. J. Virol. 78: 6122-6133 [Abstract] [Full Text]  
  • Chiu, Y.-L., Cao, H., Jacque, J.-M., Stevenson, M., Rana, T. M. (2004). Inhibition of Human Immunodeficiency Virus Type 1 Replication by RNA Interference Directed against Human Transcription Elongation Factor P-TEFb (CDK9/CyclinT1). J. Virol. 78: 2517-2529 [Abstract] [Full Text]  
  • Hermankova, M., Siliciano, J. D., Zhou, Y., Monie, D., Chadwick, K., Margolick, J. B., Quinn, T. C., Siliciano, R. F. (2003). Analysis of Human Immunodeficiency Virus Type 1 Gene Expression in Latently Infected Resting CD4+ T Lymphocytes In Vivo. J. Virol. 77: 7383-7392 [Abstract] [Full Text]  
  • Liou, L.-Y., Herrmann, C. H., Rice, A. P. (2002). Transient Induction of Cyclin T1 during Human Macrophage Differentiation Regulates Human Immunodeficiency Virus Type 1 Tat Transactivation Function. J. Virol. 76: 10579-10587 [Abstract] [Full Text]  
  • Cook, J. A., August, A., Henderson, A. J. (2002). Recruitment of Phosphatidylinositol 3-Kinase to CD28 Inhibits HIV Transcription by a Tat-Dependent Mechanism. J. Immunol. 169: 254-260 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»