This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Afonso, C. L.
Right arrow Articles by Kutish, G. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Afonso, C. L.
Right arrow Articles by Kutish, G. F.

 Previous Article  |  Next Article 

Journal of Virology, November 2001, p. 11157-11165, Vol. 75, No. 22
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.22.11157-11165.2001

Genome Sequence of a Baculovirus Pathogenic for Culex nigripalpus

C. L. Afonso,1,* E. R. Tulman,1 Z. Lu,1 C. A. Balinsky,1 B. A. Moser,2 J. J. Becnel,2 D. L. Rock,1 and G. F. Kutish1

Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York 11944,1 and Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service, U.S. Department of Agriculture, Gainesville, Florida 326042

Received 29 May 2001/Accepted 9 August 2001

In this report we describe the complete genome sequence of a nucleopolyhedrovirus that infects larval stages of the mosquito Culex nigripalpus (CuniNPV). The CuniNPV genome is a circular double-stranded DNA molecule of 108,252 bp and is predicted to contain 109 genes. Although 36 of these genes show homology to genes from other baculoviruses, their orientation and order exhibit little conservation relative to the genomes of lepidopteran baculoviruses. CuniNPV genes homologous to those from other baculoviruses include genes involved in early and late gene expression (lef-4, lef-5, lef-8, lef-9, vlf-1, and p47), DNA replication (lef-1, lef-2, helicase-1, and dna-pol), and structural functions (vp39, vp91, odv-ec27, odv-e56, p6.9, gp41, p74, and vp1054). Auxiliary genes include homologues of genes encoding the p35 antiapoptosis protein and a novel insulin binding-related protein. In contrast to these conserved genes, CuniNPV lacks apparent homologues of baculovirus genes essential (ie-1 and lef-3) or stimulatory (ie-2, lef-7, pe38) for DNA replication. Also, baculovirus genes essential or stimulatory for early-late (ie-1, ie-2), early (ie-0 and pe-38), and late (lef-6, lef-11, and pp31) gene transcription are not identifiable. In addition, CuniNPV lacks homologues of genes involved in the formation of virogenic stroma (pp31), nucleocapsid (orf1629, p87, and p24), envelope of occluded virions (odv-e25, odv-e66, odv-e18), and polyhedra (polyhedrin/granulin, p10, pp34, and fp25k). A homologue of gp64, a budded virus envelope fusion protein, was also absent, although a gene related to the other category of baculovirus budded virus envelope proteins, Ld130, was present. The absence of homologues of occlusion-derived virion (ODV) envelope proteins and occlusion body (OB) protein (polyhedrin) suggests that both CuniNPV ODV and OB may be structurally and compositionally different from those found in terrestrial lepidopteran hosts. The striking difference in genome organization, the low level of conservation of homologous genes, and the lack of many genes conserved in other baculoviruses suggest a large evolutionary distance between CuniNPV and lepidopteran baculoviruses.

* Corresponding author. Mailing address: Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944-0848. Phone: (631) 323-3011. Fax: (631) 323-3044. E-mail: cafonso{at}piadc.ars.usda.gov.

Journal of Virology, November 2001, p. 11157-11165, Vol. 75, No. 22
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.22.11157-11165.2001


This article has been cited by other articles:

  • Hilton, S., Winstanley, D. (2008). Genomic sequence and biological characterization of a nucleopolyhedrovirus isolated from the summer fruit tortrix, Adoxophyes orana. J. Gen. Virol. 89: 2898-2908 [Abstract] [Full Text]  
  • Chen, Y.-R., Wu, C.-Y., Lee, S.-T., Wu, Y.-J., Lo, C.-F., Tsai, M.-F., Wang, C.-H. (2008). Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus. J. Gen. Virol. 89: 2315-2330 [Abstract] [Full Text]  
  • Yin, F., Wang, M., Tan, Y., Deng, F., Vlak, J. M., Hu, Z., Wang, H. (2008). A Functional F Analogue of Autographa californica Nucleopolyhedrovirus GP64 from the Agrotis segetum Granulovirus. J. Virol. 82: 8922-8926 [Abstract] [Full Text]  
  • Wolff, J. L. C., Valicente, F. H., Martins, R., Oliveira, J. V. d. C., Zanotto, P. M. d. A. (2008). Analysis of the genome of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV-19) and of the high genomic heterogeneity in group II nucleopolyhedroviruses. J. Gen. Virol. 89: 1202-1211 [Abstract] [Full Text]  
  • Duffy, S. P., Becker, E. M., Whittome, B. H., Lucarotti, C. J., Levin, D. B. (2007). In vivo replication kinetics and transcription patterns of the nucleopolyhedrovirus (NeabNPV) of the balsam fir sawfly, Neodiprion abietis. J. Gen. Virol. 88: 1945-1951 [Abstract] [Full Text]  
  • Perera, O., Green, T. B., Stevens, S. M. Jr., White, S., Becnel, J. J. (2007). Proteins Associated with Culex nigripalpus Nucleopolyhedrovirus Occluded Virions. J. Virol. 81: 4585-4590 [Abstract] [Full Text]  
  • Wu, W., Lin, T., Pan, L., Yu, M., Li, Z., Pang, Y., Yang, K. (2006). Autographa californica Multiple Nucleopolyhedrovirus Nucleocapsid Assembly Is Interrupted upon Deletion of the 38K Gene. J. Virol. 80: 11475-11485 [Abstract] [Full Text]  
  • Oliveira, J. V. d. C., Wolff, J. L. C., Garcia-Maruniak, A., Ribeiro, B. M., de Castro, M. E. B., de Souza, M. L., Moscardi, F., Maruniak, J. E., Zanotto, P. M. d. A. (2006). Genome of the most widely used viral biopesticide: Anticarsia gemmatalis multiple nucleopolyhedrovirus.. J. Gen. Virol. 87: 3233-3250 [Abstract] [Full Text]  
  • Duffy, S. P., Young, A. M., Morin, B., Lucarotti, C. J., Koop, B. F., Levin, D. B. (2006). Sequence Analysis and Organization of the Neodiprion abietis Nucleopolyhedrovirus Genome. J. Virol. 80: 6952-6963 [Abstract] [Full Text]  
  • Escasa, S. R., Lauzon, H. A. M., Mathur, A. C., Krell, P. J., Arif, B. M. (2006). Sequence analysis of the Choristoneura occidentalis granulovirus genome. J. Gen. Virol. 87: 1917-1933 [Abstract] [Full Text]  
  • Lauzon, H. A. M., Garcia-Maruniak, A., de A. Zanotto, P. M., Clemente, J. C., Herniou, E. A., Lucarotti, C. J., Arif, B. M., Maruniak, J. E. (2006). Genomic comparison of Neodiprion sertifer and Neodiprion lecontei nucleopolyhedroviruses and identification of potential hymenopteran baculovirus-specific open reading frames. J. Gen. Virol. 87: 1477-1489 [Abstract] [Full Text]  
  • Katsuma, S., Daimon, T., Mita, K., Shimada, T. (2006). Lepidopteran ortholog of Drosophila breathless is a receptor for the baculovirus fibroblast growth factor.. J. Virol. 80: 5474-5481 [Abstract] [Full Text]  
  • Jakubowska, A. K., Peters, S. A., Ziemnicka, J., Vlak, J. M., van Oers, M. M. (2006). Genome sequence of an enhancin gene-rich nucleopolyhedrovirus (NPV) from Agrotis segetum: collinearity with Spodoptera exigua multiple NPV.. J. Gen. Virol. 87: 537-551 [Abstract] [Full Text]  
  • van Oers, M. M., Abma-Henkens, M. H. C., Herniou, E. A., de Groot, J. C. W., Peters, S., Vlak, J. M. (2005). Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes. J. Gen. Virol. 86: 2069-2080 [Abstract] [Full Text]  
  • Lung, O., Blissard, G. W. (2005). A Cellular Drosophila melanogaster Protein with Similarity to Baculovirus F Envelope Fusion Proteins. J. Virol. 79: 7979-7989 [Abstract] [Full Text]  
  • de Jong, J. G., Lauzon, H. A. M., Dominy, C., Poloumienko, A., Carstens, E. B., Arif, B. M., Krell, P. J. (2005). Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome. J. Gen. Virol. 86: 929-943 [Abstract] [Full Text]  
  • Lauzon, H. A. M., Jamieson, P. B., Krell, P. J., Arif, B. M. (2005). Gene organization and sequencing of the Choristoneura fumiferana defective nucleopolyhedrovirus genome. J. Gen. Virol. 86: 945-961 [Abstract] [Full Text]  
  • Li, L., Li, Q., Willis, L. G., Erlandson, M., Theilmann, D. A., Donly, C. (2005). Complete comparative genomic analysis of two field isolates of Mamestra configurata nucleopolyhedrovirus-A. J. Gen. Virol. 86: 91-105 [Abstract] [Full Text]  
  • Lauzon, H. A. M., Lucarotti, C. J., Krell, P. J., Feng, Q., Retnakaran, A., Arif, B. M. (2004). Sequence and Organization of the Neodiprion lecontei Nucleopolyhedrovirus Genome. J. Virol. 78: 7023-7035 [Abstract] [Full Text]  
  • Garcia-Maruniak, A., Maruniak, J. E., Zanotto, P. M. A., Doumbouya, A. E., Liu, J.-C., Merritt, T. M., Lanoie, J. S. (2004). Sequence Analysis of the Genome of the Neodiprion sertifer Nucleopolyhedrovirus. J. Virol. 78: 7036-7051 [Abstract] [Full Text]  
  • Saville, G. P., Patmanidi, A. L., Possee, R. D., King, L. A. (2004). Deletion of the Autographa californica nucleopolyhedrovirus chitinase KDEL motif and in vitro and in vivo analysis of the modified virus. J. Gen. Virol. 85: 821-831 [Abstract] [Full Text]  
  • Herniou, E. A., Olszewski, J. A., O'Reilly, D. R., Cory, J. S. (2004). Ancient Coevolution of Baculoviruses and Their Insect Hosts. J. Virol. 78: 3244-3251 [Abstract] [Full Text]  
  • Slack, J. M., Ribeiro, B. M., de Souza, M. L. (2004). The gp64 locus of Anticarsia gemmatalis multicapsid nucleopolyhedrovirus contains a 3' repair exonuclease homologue and lacks v-cath and ChiA genes. J. Gen. Virol. 85: 211-219 [Abstract] [Full Text]  
  • Fang, M., Wang, H., Wang, H., Yuan, L., Chen, X., Vlak, J. M., Hu, Z. (2003). Open reading frame 94 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel conserved occlusion-derived virion protein, ODV-EC43. J. Gen. Virol. 84: 3021-3027 [Abstract] [Full Text]  
  • Bideshi, D. K., Renault, S., Stasiak, K., Federici, B. A., Bigot, Y. (2003). Phylogenetic analysis and possible function of bro-like genes, a multigene family widespread among large double-stranded DNA viruses of invertebrates and bacteria. J. Gen. Virol. 84: 2531-2544 [Abstract] [Full Text]  
  • Means, J. C., Muro, I., Clem, R. J. (2003). Silencing of the Baculovirus Op-iap3 Gene by RNA Interference Reveals that It Is Required for Prevention of Apoptosis during Orgyia pseudotsugata M Nucleopolyhedrovirus Infection of Ld652Y Cells. J. Virol. 77: 4481-4488 [Abstract] [Full Text]  
  • Wu, X., Guarino, L. A. (2003). Autographa californica Nucleopolyhedrovirus orf69 Encodes an RNA Cap (Nucleoside-2'-O)-Methyltransferase. J. Virol. 77: 3430-3440 [Abstract] [Full Text]  
  • Pei, Z., Reske, G., Huang, Q., Hammock, B. D., Qi, Y., Chejanovsky, N. (2002). Characterization of the Apoptosis Suppressor Protein P49 from the Spodoptera littoralis Nucleopolyhedrovirus. J. Biol. Chem. 277: 48677-48684 [Abstract] [Full Text]  
  • Kikhno, I., Gutierrez, S., Croizier, L., Croizier, G., Ferber, M. L. (2002). Characterization of pif, a gene required for the per os infectivity of Spodoptera littoralis nucleopolyhedrovirus. J. Gen. Virol. 83: 3013-3022 [Abstract] [Full Text]  
  • Cheng, C.-H., Liu, S.-M., Chow, T.-Y., Hsiao, Y.-Y., Wang, D.-P., Huang, J.-J., Chen, H.-H. (2002). Analysis of the Complete Genome Sequence of the Hz-1 Virus Suggests that It Is Related to Members of the Baculoviridae. J. Virol. 76: 9024-9034 [Abstract] [Full Text]  
  • Pearson, M. N., Rohrmann, G. F. (2002). Transfer, Incorporation, and Substitution of Envelope Fusion Proteins among Members of the Baculoviridae, Orthomyxoviridae, and Metaviridae (Insect Retrovirus) Families. J. Virol. 76: 5301-5304 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»